First Report of Fusarium redolens Causing Fusarium Yellowing and Wilt of Chickpea in Tunisia
2017; American Phytopathological Society; Volume: 101; Issue: 6 Linguagem: Inglês
10.1094/pdis-08-16-1114-pdn
ISSN1943-7692
AutoresMariem Bouhadida, Warda Jendoubi, Samia Gargouri, Mohamed Béji, Mohamed Kharrat, W. Chen,
Tópico(s)Plant Disease Resistance and Genetics
ResumoHomePlant DiseaseVol. 101, No. 6First Report of Fusarium redolens Causing Fusarium Yellowing and Wilt of Chickpea in Tunisia PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Fusarium redolens Causing Fusarium Yellowing and Wilt of Chickpea in TunisiaM. Bouhadida, W. Jendoubi, S. Gargouri, M. Beji, M. Kharrat, and W. ChenM. Bouhadida, W. Jendoubi, S. Gargouri, M. Beji, M. Kharrat, and W. ChenAffiliationsAuthors and Affiliations M. Bouhadida , Field Crops Laboratory, University of Carthage, INRAT, 2080 Ariana, Tunisia W. Jendoubi , Field Crops Laboratory, University of Carthage, INRAT, 2080 Ariana, Tunisia; and National Agronomic Institute of Tunisia, 1082 Tunis, Tunisia S. Gargouri , Plant Protection Laboratory, University of Carthage, INRAT, 2080 Ariana, Tunisia M. Beji , Field Crops Laboratory, University of Carthage, INRAT, 2080 Ariana, Tunisia; and University of Tunis El Manar, Faculty of Sciences of Tunis, Campus University, El Manar, Tunis, Tunisia M. Kharrat , Field Crops Laboratory, University of Carthage, INRAT, 2080 Ariana, Tunisia W. Chen , USDA-ARS, Washington State University, Pullman, 99164. Published Online:4 Apr 2017https://doi.org/10.1094/PDIS-08-16-1114-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Chickpea is an important food crop in Tunisia. Chickpea plants showing wilt symptoms in Tunisia have been attributed in the past solely to race 0 of Fusarium oxysporum f. sp. ciceris (Foc). However, Tunisian chickpea cultivars, such as Béja 1, known to be resistant to race 0 of Foc have shown wilting symptoms since 2010. To ascertain the race or identity of species responsible for the disease, chickpea plants showing yellowing and/or vascular brown discoloration were collected from northwestern Tunisia in 2013. Stem pieces (3 cm) of symptomatic plants were surface disinfested and plated on water agar (2% agar in water). Ninety-five single-spore isolates each from a separate plant were characterized on potato dextrose agar and on carnation leaf agar. They are all morphologically similar to F. oxysporum (Leslie and Summerell 2006), and were further characterized using PCR with species-specific primers. Among them, 67 isolates (70.5%) were identified as F. oxysporum using FO-PCR primers of Mishra et al. (2003); however, none of them could be assigned to Foc using the FOC-PCR primers of Jiménez-Gasco and Jiménez-Díaz (2003). The remaining 28 (29.5%) isolates were identified as F. redolens based on PCR using the F. redolens-specific (FR)-PCR assay of Bogale et al. (2007). Three isolates, TNFR-23, TNFR-76, and TNFR-114 of F. redolens, were further confirmed by the TEF-1α gene sequences using primers EF1 (5′-ATGGGTAAGGA(A/G)GACAAGAC-3′) and EF2 (5′-GGA(G/A)GTACCAGT(G/C)ATCATG-TT-3′). Sequences of these three isolates (GenBank accession nos. KU872083, KU872082, and KU872081, respectively) displayed 99% homology to accession KP267485.1 of F. redolens, and more than 99% similarity to accession FD_01081_EF-1a of F. redolens of the FUSARIUM-ID database. Morphological characteristics were consistent with descriptions of F. redolens (Leslie and Summerell 2006): relatively flat aerial mycelium with white to pink color, abundant chlamydospores, 3 to 5 septated macroconidia (26.7 to 31.2 μm) in sporodochia, oval or cylindrical microconidia (6.2 to 8.7 μm) in false heads. Four isolates of F. redolens were tested for pathogenicity on three chickpea genotypes (ILC482, JG62, and cv. Béja 1). Chickpea seedlings at the four-leaf stage were inoculated by dipping trimmed roots in spore suspension of 106 micro- and macroconidia/ml. Inoculated plants were planted in greenhouse with 10 plants as one replicate (three replicates per treatment) and maintained at 25°C and 12-h photoperiod. Plants dipped into water were used as controls. Disease incidence was evaluated four times weekly starting 2 weeks after inoculation. The experiment was performed three times. Disease symptoms were similar to those observed in the field. Up to 100% disease incidence occurred on chickpea lines ILC482 and cv. Béja 1, and line JG62 appeared to be the most resistant with only 20% disease incidence. Isolates recovered from inoculated plants were identified as F. redolens using FR-PCR, fulfilling Koch's postulates. Chickpea wilt caused by F. redolens was previously reported in Lebanon, Morocco, Pakistan, and Spain (Jiménez-Fernández et al. 2011). This is the first report of F. redolens causing wilting on chickpea in Tunisia. There is no report of F. redolens causing diseases on other crops in Tunisia. Thus, there is a need to clarify the biology and ecology of this fungus to provide adequate information regarding its economic significance and develop appropriate control measures.References:Bogale, M., et al. 2007. FEMS Microbiol. Lett. 271:27. https://doi.org/10.1111/j.1574-6968.2007.00687.x Crossref, ISI, Google ScholarJiménez-Fernández, D., et al. 2011. Plant Dis. 95:860. https://doi.org/10.1094/PDIS-12-10-0946 Link, ISI, Google ScholarJiménez-Gasco, M. M., and Jiménez-Díaz, R. M. 2003. Phytopathology 93:200. https://doi.org/10.1094/PHYTO.2003.93.2.200 Link, ISI, Google ScholarLeslie, J. F., and Summerell, B. A., eds. 2006. The Fusarium Laboratory Manual. Blackwell Publishing, Ames, IA. doi:10.1002/9780470278376 Crossref, Google ScholarMishra, P. K., et al. 2003. FEMS Microbiol. Lett. 218:329. https://doi.org/10.1111/j.1574-6968.2003.tb11537.x Crossref, ISI, Google ScholarM. Bouhadida and W. Jendoubi contributed equally.DetailsFiguresLiterature CitedRelated Vol. 101, No. 6 June 2017SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 17 May 2017Published: 4 Apr 2017First Look: 27 Feb 2017Accepted: 19 Feb 2017 Pages: 1038-1038 InformationThis article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 2017.Cited byFirst Report of Wilt on Lentil (Lens culinaris) Caused by Fusarium redolens in TunisiaS. Chekali, A. Ouji, S. Somma, M. Masiello, W. Douihech, E. Khemir, A. Moretti, and S. 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