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Amino-functional Silsesquioxanes (POSS)-Effective Glass Surface Modifiers in Solidphase Nucleic Acid Synthesis

2017; Bentham Science Publishers; Volume: 21; Issue: 25 Linguagem: Inglês

10.2174/1385272821666170222111024

1875-5348

Karol Szubert, Marta Piepiorka, Magdalena Jankowska‐Wajda, Jolanta Brzezińska, Karol Pasternak, Natalia Remszel, Emilia Frydrych‐Tomczak, Hieronim Maciejewski, Marcin K. Chmielewski,

Synthesis and characterization of novel inorganic/organometallic compounds

Branched amino functional polyoctahedral silsesquioxanes (POSS) were prepared by using different stoichiometric ratios of two olefins: allylamine and vinyltrimethoxysilane. Amino-rich CPG particles were produced by direct silanisation with amino-POSS silanes. The parameters of surface morphology (total volume and size of pores) of the obtained supports were determined by Brunauer-Emmett-Teller method. Results from Scanning Electron Microscopy show that there is no alteration in surface chemistry and only modest changes in mechanical properties are detected. The POSS-modified CPG supports were demonstrated as effective scaffolds for the chemical synthesis of nucleic acids. Keywords: Silsesquioxane, solid-phase synthesis, nucleic acids, RNA or DNA synthesis, solid supports, scanning electron microscopy.