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An Effective and Potentially Safe Blood Disinfection Protocol Using Tetrapyrrolic Photosensitizers

2017; Future Science Ltd; Volume: 9; Issue: 4 Linguagem: Inglês

10.4155/fmc-2016-0217

1756-8927

Lúcia Marciel, Luís Teles, Beatriz Moreira, Mário Pacheco, Leandro M. O. Lourenço, Maria G. P. M. S. Neves, João P. C. Tomé, M. Amparo F. Faustino, Adelaide Almeida,

Innovative Microfluidic and Catalytic Techniques Innovation

Conventional disinfection techniques, considered safe for plasma, are usually associated with collateral damages on concentrated platelets and erythrocytes. Alternative methods are required and antimicrobial photodynamic therapy (aPDT) seems promising. In this study the effectiveness of two photosensitizers (PS), a porphyrin and a phthalocyanine, to disinfect blood products was evaluated.The cationic porphyrin was more effective in the photoinactivation of bacteria. Also, no significant osmotic stress was found for samples treated with PS at 5.0 µM in isotonic conditions after antimicrobial photodynamic therapy.Effective reduction of Gram-positive bacteria at 5.0 µM of PS provided promising indications toward its safe use to disinfect blood samples. For Gram-negative bacteria, lower PS concentrations, between 5.0 and 10 µM, must be tested.