Anthracnose of Persimmon ( Diospyros kaki ) Caused by Colletotrichum horii in Sangju, Korea
2017; American Phytopathological Society; Volume: 101; Issue: 6 Linguagem: Inglês
10.1094/pdis-01-17-0085-pdn
ISSN1943-7692
AutoresJong Yeob Jeon, Oliul Hassan, Taehyun Chang, D. W. Lee, J. S. Shin, Nam Kwon Oh,
Tópico(s)Mycorrhizal Fungi and Plant Interactions
ResumoHomePlant DiseaseVol. 101, No. 6Anthracnose of Persimmon (Diospyros kaki) Caused by Colletotrichum horii in Sangju, Korea PreviousNext DISEASE NOTES OPENOpen Access licenseAnthracnose of Persimmon (Diospyros kaki) Caused by Colletotrichum horii in Sangju, KoreaJ. Y. Jeon, O. Hassan, T. Chang, D. W. Lee, J. S. Shin, and N. K. OhJ. Y. Jeon, O. Hassan, T. Chang, D. W. Lee, J. S. Shin, and N. K. OhAffiliationsAuthors and Affiliations J. Y. Jeon O. Hassan T. Chang , Division of Ecology and Environmental System, College of Ecology and Environmental Sciences, Kyungpook National University, Sangju-si, Gyeongsangbuk-do, 37224, Republic of Korea D. W. Lee , Department of Ecological Science, Kyungpook National University, Sangju, Gyeongbuk, 37224, Republic of Korea J. S. Shin N. K. Oh , Division of Ecology and Environmental System, College of Ecology and Environmental Sciences, Kyungpook National University, Sangju-si, Gyeongsangbuk-do, 37224, Republic of Korea. Published Online:3 Apr 2017https://doi.org/10.1094/PDIS-01-17-0085-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Sangju, South Korea, is famous worldwide for dried persimmon (gotgam) and contributes 60% to the nation's total production of persimmon. However, one of the main problems in cultivating persimmon is anthracnose disease, which results in huge economic losses. Colletotrichum species such as Colletotrichum gloeosporioides, C. acutatum, and C. horii are implicated in causing persimmon anthracnose in Korea (Kwon et al. 2011, 2013). In 2013, diseased fruits showing typical anthracnose symptoms such as circular, sunken, and dark black spots were collected from an orchard in Sangju, and the presumed causative agents were isolated from them. Diseased tissue (1 cm2) was cut aseptically from inside of each lesion, surface-disinfected in 0.5% sodium hypochlorite solution for 2 min followed by three washes with sterile distilled water, subsequently placed onto potato dextrose agar (PDA) amended with streptomycin (0.05 g/liter), and then incubated at 25°C in the dark for 3 days. To obtain pure cultures, hyphal tips were transferred onto a fresh PDA plate. Morphological characteristics were monitored to identify the causal agent. Seven-day-old colonies were white to gray in color with conidia developing across colonies. The mycelia were hyaline and septate. Conidia were cylindrical, straight or slightly curved with round ends, single celled, and 14.5 to 22.5 × 4 to 6.5 μm (avg. 17 × 4.5 μm) (n = 25). Appressoria were short-cylindric, 9.3 to 13.8 × 5.0 to 8.6 μm (avg. 11.0 × 6.8 μm), and brown. The morphological characteristics of our isolates were mostly similar to those of Colletotrichum species in C. gloeosporioides species complex including C. horii (Weir and Johnston 2010; Weir et al. 2012). Genomic DNA was extracted from the isolates for molecular analysis. The internal transcribed spacer (ITS), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and actin (ACT) regions of genomic DNA were amplified using primer pairs ITS-1F/ITS4, GDF/GDR, and ACT-512F/ACT-783R, respectively. BLAST search analyses based on ITS gene sequence of the present isolates (LC186039–42) showed 100% similarity with the sequence from C. horii isolate OGaC1 (GenBank KU239182), ACT (LC186031–34) showed 100% similarity with the sequence from C. horii strain CPC 20992 (KC566947), and GAPDH (LC186035–38) showed 100% similarity with the sequence from C. horii strain C1069 (JX010001). Based on the combined dataset of ITS, ACT, and GAPDH, a molecular phylogenic analysis was performed using the neighbor-joining method in MEGA6. The studied isolates formed a clade with reliable C. horii strain relatives from GenBank with 100% bootstrap support. To confirm the pathogenicity of our isolates, pathogenicity tests were performed on mature persimmon fruits. Conidial suspension (105 conidia/ml of ICK-111) was sprayed on the fruits (disinfected) until the suspension ran off. The fruits were placed in a sterile plastic box and incubated at 25°C in the dark. After 3 to 5 days of inoculation, black pin-headed spots were observed on the surface of inoculated fruits. The fungus was successfully reisolated and identified as C. horii (confirming Koch's postulates). C. horii has been reported to cause anthracnose of persimmon in Gyeongnam Province of southern Korea, New Zealand, and Brazil (Kwon et al. 2011, 2013; May de Mio et al. 2015; Weir et al. 2012). To our knowledge, this is the first report of C. horii causing anthracnose of persimmon (Diospyros kaki L. f.) in Gyeongsangbuk-do Province and the second in South Korea.References:Kwon, J.-H., et al. 2011. Plant Pathol. J. 27:100. https://doi.org/10.5423/PPJ.2011.27.1.100 Crossref, ISI, Google ScholarKwon, J.-H., et al. 2013. J. Phytopathol. 161:497. https://doi.org/10.1111/jph.12096 Crossref, ISI, Google ScholarMay de Mio, L. L., et al. 2015. Rev. Bras. Frutic. 37:256. https://doi.org/10.1590/0100-2945-044/14 Crossref, ISI, Google ScholarWeir, B. S., et al. 2012. Stud. Mycol. 73:115. https://doi.org/10.3114/sim0011 Crossref, ISI, Google ScholarWeir, B. S., and Johnston, P. R. 2010. Mycotaxon 111:209. https://doi.org/10.5248/111.209 Crossref, ISI, Google ScholarThese authors contributed equally to this work. The authors declare no conflict of interest.DetailsFiguresLiterature CitedRelated Vol. 101, No. 6 June 2017SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 17 May 2017Published: 3 Apr 2017First Look: 27 Feb 2017Accepted: 20 Feb 2017 Pages: 1035-1035 Information© 2017 The American Phytopathological SocietyCited byIdentification of New Sources of Resistance to Anthracnose Caused by Colletotrichum horii among Persimmon Germplasms21 February 2022 | Horticulturae, Vol. 8, No. 2Colletotrichum species and complexes: geographic distribution, host range and conservation status29 September 2021 | Fungal Diversity, Vol. 110, No. 1Morphological characterization and molecular identification of Colletotrichum species associated to sweet persimmon anthracnose in Southern Brazil1 January 2021 | Ciência Rural, Vol. 51, No. 9Persimmon anthracnose: a comparative study of aggressiveness on shoot and fruit among Colletotrichum horii isolates in southern Brazil1 January 2020 | Ciência Rural, Vol. 50, No. 10Colletotrichum Species Associated with Japanese Plum (Prunus salicina) Anthracnose in South Korea19 August 2019 | Scientific Reports, Vol. 9, No. 1Colletotrichum kakivorum sp. nov., a new leaf spot pathogen of persimmon in Korea30 July 2018 | Mycological Progress, Vol. 17, No. 10Sensitivity Variation to Demethylation Inhibiting Fungicides of Colletotrichum horii, Isolated Anthracnose Pathogens from Persimmon Tree in Sangju and YeongdongThe Korean Journal of Pesticide Science, Vol. 22, No. 3Molecular and Morphological Characterization of Colletotrichum Species in the Colletotrichum gloeosporioides Complex Associated with Persimmon Anthracnose in South KoreaOliul Hassan, Jong Yeob Jeon, Taehyun Chang, Jun Sung Shin, Nam Kwon Oh, and Yong Se Lee23 March 2018 | Plant Disease, Vol. 102, No. 5In vitro Antifungal Activities of Fungicides against Japanese Plum Fruit Anthracnose FungiKorean Journal of Environmental Agriculture, Vol. 37, No. 1Isolation and Characterization of Colletotrichum Isolates Causing Anthracnose of Japanese Plum FruitKorean Journal of Environmental Agriculture, Vol. 36, No. 4Responses of Colletotrichum horii Isolated from Persimmon in Sangju and Yeongdong to Carbendazim and Thiophanate-methylThe Korean Journal of Pesticide Science, Vol. 21, No. 4
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