Centipede venom peptide SsmTX-I with two intramolecular disulfide bonds shows analgesic activities in animal models
2017; Wiley; Volume: 23; Issue: 5 Linguagem: Inglês
10.1002/psc.2988
ISSN1099-1387
AutoresYing Wang, Xiaojie Li, Meifeng Yang, Chunyun Wu, Zhirong Zou, Jing Tang, Xinwang Yang,
Tópico(s)Pain Mechanisms and Treatments
ResumoJournal of Peptide ScienceVolume 23, Issue 5 p. 384-391 Research Article Centipede venom peptide SsmTX-I with two intramolecular disulfide bonds shows analgesic activities in animal models Ying Wang, Ying Wang Ethic Drug Screening and Pharmacology Center, Key Laboratory of Chemistry in Ethnic Medicine Resource, State Ethnic Affairs Commission and Ministry of Education, Yunnan University of Nationalities, Kunming, 650500 ChinaThese two authors contributed equally to this work.Search for more papers by this authorXiaojie Li, Xiaojie Li Department of Biochemistry and Molecular Biology, Faculty of Basic Medical Science, Kunming Medical University, Kunming, 650500 ChinaThese two authors contributed equally to this work.Search for more papers by this authorMeifeng Yang, Meifeng Yang orcid.org/0000-0003-3210-8908 Department of Biochemistry and Molecular Biology, Faculty of Basic Medical Science, Kunming Medical University, Kunming, 650500 ChinaSearch for more papers by this authorChunyun Wu, Chunyun Wu Department of Anatomy and Histology and Embryology, Faculty of Basic Medical Science, Kunming Medical University, Kunming, 650500 ChinaSearch for more papers by this authorZhirong Zou, Zhirong Zou Department of Anatomy and Histology and Embryology, Faculty of Basic Medical Science, Kunming Medical University, Kunming, 650500 ChinaSearch for more papers by this authorJing Tang, Corresponding Author Jing Tang gracett916@163.com Department of Biochemistry and Molecular Biology, Faculty of Basic Medical Science, Kunming Medical University, Kunming, 650500 ChinaCorrespondence to: Xinwang Yang and Jing Tang, Faculty of Basic Medical Science, Kunming Medical University, 1168 West Chunrong Road, Kunming, Yunnan 650500, China. E-mail: yangxinwanghp@163.com; gracett916@163.comSearch for more papers by this authorXinwang Yang, Corresponding Author Xinwang Yang yangxinwanghp@163.com Department of Anatomy and Histology and Embryology, Faculty of Basic Medical Science, Kunming Medical University, Kunming, 650500 ChinaCorrespondence to: Xinwang Yang and Jing Tang, Faculty of Basic Medical Science, Kunming Medical University, 1168 West Chunrong Road, Kunming, Yunnan 650500, China. E-mail: yangxinwanghp@163.com; gracett916@163.comSearch for more papers by this author Ying Wang, Ying Wang Ethic Drug Screening and Pharmacology Center, Key Laboratory of Chemistry in Ethnic Medicine Resource, State Ethnic Affairs Commission and Ministry of Education, Yunnan University of Nationalities, Kunming, 650500 ChinaThese two authors contributed equally to this work.Search for more papers by this authorXiaojie Li, Xiaojie Li Department of Biochemistry and Molecular Biology, Faculty of Basic Medical Science, Kunming Medical University, Kunming, 650500 ChinaThese two authors contributed equally to this work.Search for more papers by this authorMeifeng Yang, Meifeng Yang orcid.org/0000-0003-3210-8908 Department of Biochemistry and Molecular Biology, Faculty of Basic Medical Science, Kunming Medical University, Kunming, 650500 ChinaSearch for more papers by this authorChunyun Wu, Chunyun Wu Department of Anatomy and Histology and Embryology, Faculty of Basic Medical Science, Kunming Medical University, Kunming, 650500 ChinaSearch for more papers by this authorZhirong Zou, Zhirong Zou Department of Anatomy and Histology and Embryology, Faculty of Basic Medical Science, Kunming Medical University, Kunming, 650500 ChinaSearch for more papers by this authorJing Tang, Corresponding Author Jing Tang gracett916@163.com Department of Biochemistry and Molecular Biology, Faculty of Basic Medical Science, Kunming Medical University, Kunming, 650500 ChinaCorrespondence to: Xinwang Yang and Jing Tang, Faculty of Basic Medical Science, Kunming Medical University, 1168 West Chunrong Road, Kunming, Yunnan 650500, China. E-mail: yangxinwanghp@163.com; gracett916@163.comSearch for more papers by this authorXinwang Yang, Corresponding Author Xinwang Yang yangxinwanghp@163.com Department of Anatomy and Histology and Embryology, Faculty of Basic Medical Science, Kunming Medical University, Kunming, 650500 ChinaCorrespondence to: Xinwang Yang and Jing Tang, Faculty of Basic Medical Science, Kunming Medical University, 1168 West Chunrong Road, Kunming, Yunnan 650500, China. E-mail: yangxinwanghp@163.com; gracett916@163.comSearch for more papers by this author First published: 01 March 2017 https://doi.org/10.1002/psc.2988Citations: 16 Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract Pain is a major symptom of many diseases and results in enormous pressures on human body or society. Currently, clinically used analgesic drugs, including opioids and nonsteroidal anti-inflammatory drugs, have adverse reactions, and thus, the development of new types of analgesic drug candidates is urgently needed. Animal venom peptides have proven to have potential as new types of analgesic medicine. In this research, we describe the isolation and characterization of an analgesic peptide from the crude venom of centipede, Scolopendra subspinipes mutilans. The amino acid sequence of this peptide was identical with SsmTX-I that was previously reported as a specific Kv2.1 ion channel blocker. Our results revealed that SsmTX-I was produced by posttranslational processing of a 73-residue prepropeptide. The intramolecular disulfide bridge motifs of SsmTX-I was Cys1–Cys3 and Cys2–Cys4. Functional assay revealed that SsmTX-I showed potential analgesic activities in formalin-induced paw licking, thermal pain, and acetic acid-induced abdominal writhing mice models. Our research provides the first report of cDNA sequences, disulfide motif, successful synthesis, and analgesic potential of SsmTX-I for the development of pain-killing drugs. It indicates that centipede peptide toxins could be a treasure trove for the search of novel analgesic drug candidates. Copyright © 2017 European Peptide Society and John Wiley & Sons, Ltd. Citing Literature Volume23, Issue5May 2017Pages 384-391 RelatedInformation
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