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Hormonemate Derivatives from Dothiora sp., an Endophytic Fungus

2017; American Chemical Society; Volume: 80; Issue: 4 Linguagem: Inglês

10.1021/acs.jnatprod.6b00680

1520-6025

Mercedes Pérez-Bonilla, Víctor González-Menéndez, Ignacio Pérez‐Victoria, Nuria de Pedro, Jesús Martı́n, Joaquı́n Molero Mesa, Manuel Casares-Porcel, M. Reyes González-Tejero, Francisca Vicente, Olga Genilloud, José R. Tormo, Fernando Reyes,

Plant Pathogens and Fungal Diseases

A search for cytotoxic agents from cultures of the endophytic fungus Dothiora sp., isolated from the endemic plant Launaea arborescens, led to the isolation of six new compounds structurally related to hormonemate, with moderate cytotoxic activity against different cancer cell lines. By using a bioassay-guided fractionation approach, hormonemates A–D (1–4), hormonemate (5), and hormonemates E (6) and F (7) were obtained from the acetone extract of this fungus. Their structures were determined using a combination of HRMS, ESI-qTOF-MS/MS, 1D and 2D NMR experiments, and chemical degradation. The cytotoxic activities of these compounds were evaluated by microdilution colorimetric assays against human breast adenocarcinoma (MCF-7), human liver cancer cells (HepG2), and pancreatic cancer cells (MiaPaca_2). Most of the compounds displayed cytotoxic activity against this panel.