A mitochondria-targetable fluorescent probe for ratiometric detection of SO2 derivatives and its application in live cell imaging
2017; Elsevier BV; Volume: 247; Linguagem: Inglês
10.1016/j.snb.2017.03.032
ISSN1873-3077
AutoresLijun Tang, Ping He, Xiaomei Yan, Jiazheng Sun, Keli Zhong, Shuhua Hou, Yanjiang Bian,
Tópico(s)Nitric Oxide and Endothelin Effects
ResumoA fluorescent probe (DBT) based on conjugation of 2,3-dimethylbenzo[d]thiazol-3-ium iodide to 3-(benzo[d]thiazol-2-yl)-2-hydroxy-5-methylbenzaldehyde for the detection of sulfur dioxide derivatives (HSO3−/SO32−) has been developed. DBT exhibits highly selective colorimetric and ratiometric fluorescence dual response to HSO3− with a fluorescence detection limit of 3.50 × 10−7 M. The HSO3− sensing mechanism was confirmed to undergo nucleophilic addition of HSO3− to alkene double bond of DBT as evidenced by 1H NMR and HRMS analysis. The probe is MCF-7 cell permeable and applicable for fluorescence imaging of exogenous and intrinsically generated intracellular SO2 derivatives in the living cells. Fluorescence co-localization studies reveal that DBT is a mitochondria-targetable fluorescent probe. Thus, DBT has potential application for exploring the role played by SO2 derivatives in biology.
Referência(s)