Recombination in bacteriophage λ
1968; Elsevier BV; Volume: 34; Issue: 2 Linguagem: Inglês
10.1016/0022-2836(68)90251-9
ISSN1089-8638
Autores Tópico(s)Monoclonal and Polyclonal Antibodies Research
ResumoTwo types of recombination-deficient (red−) λ mutants have been studied: point mutants isolated after hydroxylamine mutagenesis, and certain deletion mutants isolated as plaque-forming biotin-transducing strains (λbio). In a recombinationd-eficient (rec−) host, crosses of the λbio deletions, or the least leaky point mutant, give recombinants at less than 1100 the frequency of the wild-type. In a rec+ host, recombination is restored to 110 to 12 the wild-type frequency, depending on the particular mutant. The product of at least one red gene is diffusible. All the mutant sites lie on the A map between the int and cIII loci. These results demonstrate that λ produces at least one component of the system responsible for general vegetative phage recombination, and that the bacterial recombination system can replace this phage function.
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