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First Report of Colletotrichum siamense Causing Anthracnose on Acca sellowiana Fruits in Brazil
2017; American Phytopathological Society; Volume: 101; Issue: 6 Linguagem: Inglês
10.1094/pdis-01-17-0096-pdn
ISSN1943-7692
AutoresVinícius Spolaor Fantinel, Marlove Fátima Brião Muniz, Elena Blume, Maristela Machado Araújo, Tales Poletto, T. T. da Silva, Adriana Falcão Dutra, Caciara Gonzatto Maciel, Ricardo Harakava,
Tópico(s)Plant Pathogenic Bacteria Studies
ResumoHomePlant DiseaseVol. 101, No. 6First Report of Colletotrichum siamense Causing Anthracnose on Acca sellowiana Fruits in Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Colletotrichum siamense Causing Anthracnose on Acca sellowiana Fruits in BrazilV. S. Fantinel, M. F. B. Muniz, E. Blume, M. M. Araújo, T. Poletto, T. T. da Silva, A. F. Dutra, C. G. Maciel, and R. HarakavaV. S. Fantinel, M. F. B. Muniz, E. Blume, M. M. Araújo, T. Poletto, T. T. da Silva, A. F. Dutra, C. G. Maciel, and R. HarakavaAffiliationsAuthors and Affiliations V. S. Fantinel M. F. B. Muniz E. Blume M. M. Araújo T. Poletto T. T. da Silva A. F. Dutra C. G. Maciel , Departamento de Fitopatologia da Universidade Federal de Santa Maria, Brasil R. Harakava , Biological Institute of São Paulo, Brazil. Published Online:6 Apr 2017https://doi.org/10.1094/PDIS-01-17-0096-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Acca sellowiana (Berg) Burret is a fruit tree (feijoa) of economic importance due to the high organoleptic potential of the fruits. In Brazil, A. sellowiana is cultivated in the southern region on approximately 100 ha distributed among several small farms with annual production of 20,000 kg. In March of 2012, fruits with symptoms of anthracnose were observed from orchards of the municipality of São Pedro do Sul (29°59′32″ S, 54°10′84″ W), Rio Grande do Sul (RS) state, Brazil. The disease caused irregularly shaped, well defined dark spots that, after increasing in size, became depressed and coalesced, resulting in rotten fruit. Light pink acervuli were observed on these diseased fruits. The disease affected approximately 75% of the fruits in the orchards and became more intense postharvest. Twenty diseased fruits were collected in the municipality of São Pedro do Sul, RS, from a single orchard. These fruits were surface sterilized with 70% ethanol for 30 s, then in 1% sodium hypochlorite for 5 min, and rinsed three times in sterilized distilled water. Fruits were placed in a moist chamber and incubated at 25 ± 1°C with a photoperiod of 12 h for 5 days. The pure culture was obtained through single spore isolation and cultivated in V8 medium at 25 ± 1°C for 8 days and 12 h photoperiod. The colony showed dark green coloration with white aerial mycelium and daily average growth of 9 mm in diameter. The conidia were hyaline, without septa, spindle-shaped with obtuse ends, smooth walls, guttulate, measuring from 7.5 to 17.5 × 2.5 to 7.5 μm, which was compatible with the morphological characteristics of the Colletotrichum gloeosporioides complex. The elongation factor genes 1-α (TEF1-α), β-tubulin (βTUB), and actin (ACT) were amplified and sequenced. The gene sequences of TEF1-α (KX845557), βTUB (KX858806), and ACT (KX858807) were deposited in GenBank and had similarities of 99%, 100%, and 100% with C. siamense KC 790614, KU 252165, and JX 009435, respectively. A pathogenicity test was carried out to fulfill the Koch's postulate. Twenty healthy fruits of intermediate stage of development were inoculated with a spore suspension (106 conidia ml–1), where each fruit received 100 μl divided in eight drops distributed separately. In the control treatment, fruits were inoculated with sterilized water. The fruits were placed in sterilized plastic boxes with two moist sheets of filter paper and incubated at 25 ± 1°C with a photoperiod of 12 h. The test was repeated three times. Disease symptoms were observed on inoculated fruits after 4 days. The pathogen was reisolated from the injuries and identified as C. siamense belonging to the C. gloeosporioides complex. In Brazil, C. siamense has been reported on Coffea arabica (coffee) (Silva et. al. 2012) and Fragaria × ananassa (strawberry) (Capobiango et. al. 2016), but to our knowledge this is the first report of this pathogen causing rotting of fruits (anthracnose) in A. sellowiana production in Brazil.References:Capobiango, N. P., et al. 2016. Plant Dis. 100:859. https://doi.org/10.1094/PDIS-10-15-1121-PDN Link, ISI, Google ScholarSilva, D. N., et al. 2012. Mycologia 104:396. https://doi.org/10.3852/11-145 Crossref, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 101, No. 6 June 2017SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 17 May 2017Published: 6 Apr 2017First Look: 6 Mar 2017Accepted: 1 Mar 2017 Pages: 1035-1035 Information© 2017 The American Phytopathological SocietyCited byBioactivity of Cupressus sempervirens and Cupressus lusitanica leaf essential oils on Colletotrichum fructicola22 July 2022 | Journal of Essential Oil Research, Vol. 35, No. 1Pest categorisation of Colletotrichum aenigma, C. alienum, C. perseae, C. siamense and C. theobromicolaEFSA Journal, Vol. 20, No. 8Multifunctional Role of Acca sellowiana from Farm Management to Postharvest Life: A Review29 July 2022 | Agronomy, Vol. 12, No. 8Colletotrichum species associated with sugarcane red rot in BrazilFungal Biology, Vol. 126, No. 4Colletotrichum siamenseCABI Compendium, Vol. CABI CompendiumColletotrichum species and complexes: geographic distribution, host range and conservation status29 September 2021 | Fungal Diversity, Vol. 110, No. 1First Report of Colletotrichum siamense Causing Blossom Blight on Thai Basil (Ocimum basilicum) in MalaysiaS. 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