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Microfluidic high performance liquid chromatography-chip hyphenation to inductively coupled plasma–mass spectrometry

2017; Elsevier BV; Volume: 1497; Linguagem: Inglês

10.1016/j.chroma.2017.03.025

1873-3778

David Bishop, Lucas Blanes, Alexander B. Wilson, Thor Wilbanks, Kevin Killeen, Rudolf Grimm, Ross Wenzel, Derek Major, Mirek Macka, David J. Clarke, Robin Schmid, Nerida Cole, Philip Doble,

Metabolomics and Mass Spectrometry Studies

The Agilent Chip Cube Interface is a microfluidic chip-based technology originally designed for nanospray molecular mass spectrometry in which the sample enrichment, nano-column, tubing, connectors and spray tip were integrated into a single biocompatible chip. Here we describe the hyphenation of the Chip Cube Interface to ICP-MS via modification of the standard HPLC chip design and a new total consumption nebuliser suitable for flow rates as low as 300 nL min−1. The potential of the instrument to eliminate common nanoLC – ICP-MS shortcomings such as leaks, blockages and band-broadening was demonstrated via analysis of cyanocobalamin in equine plasma. The method was linear over three orders of magnitude with an r2 of 0.9999, the peak area repeatability was 1.9% (n = 7), and the detection limit was 14 ng mL−1. This novel configuration of the Chip Cube Interface coupled to ICP-MS is a suitable platform for the analysis of biomolecules associated with trace metals and speciation applications.