Caracterizacion genómica mediante AFLP de Stevia rebaudiana Bertoni cultivada en el departamento de Antioquia (Colombia)
2007; University "Marta Abreu" of Las Villas; Volume: 7; Issue: 2 Linguagem: Inglês
ISSN
1609-1841
AutoresDiego Martínez, Luís Rojas, Aura Urreal, Elio Jiménez, Zulma Monsalve F,
Tópico(s)Biochemical Analysis and Sensing Techniques
ResumoStevia rebaudiana Bertoni, Asteraceae family (2n = 22), is a plant native from Paraguay, it has in leaves tissues a high production of some substances with strong edulcorant power, getting to be 320 sweet times but that the sugar. Stevia was introduced, in Department of Antioquia, Colombia, at different times from diverse places. Its cultivation has been spread in the Department, additionally is an alternative for illicit cultivated crops substitution. AFLP marker were generated from genomic DNA of two reference varieties (‘Morita’ and ‘Bertoni’) and samples from fields crops located in different regions of Department of Antioquia. Ten AFLP primer combinations revealed 923 bands and 39.22% polymorphism. Dice coefficient similarity analysis show differences between both ‘Morita’ and ‘Bertoni’ varieties. Primer combination Eco RI-AGG/ Tru 9I-ATG, was able to identify every of two cultivars, and established two groups for Department of Antioquia Stevia varieties. Samples from Tamesis, Andes, Jerico, San Jose del Nus and Santa Fe de Antioquia localities were grouped within ‘Morita’ cultivar and samples from Fredonia, Cisneros localities and University of Antioquia laboratories were grouped within ‘Bertoni’ variety. Key words: ‘Bertoni’, molecular marker, ‘Morita’
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