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A Reversible Fluorescent Probe for Real‐Time Quantitative Monitoring of Cellular Glutathione

2017; Wiley; Volume: 56; Issue: 21 Linguagem: Inglês

10.1002/anie.201702114

1521-3773

Zhixue Liu, Xin Zhou, Miao Yu, Ying Hu, Nahyun Kwon, Xue Wu, Juyoung Yoon,

Molecular Sensors and Ion Detection

Abstract The ability to monitor and quantify glutathione (GSH) in live cells is essential in order to gain a detailed understanding of GSH‐related pathological events. However, owing to their irreversible response mechanisms, most existing fluorescent GSH probes are not suitable for this purpose. We have developed a ratiometric fluorescent probe (QG‐ 1 ) for quantitatively monitoring cellular GSH. The probe responds specifically and reversibility to GSH with an ideal dissociation constant ( K d ) of 2.59 m m and a fast response time ( t 1/2 =5.82 s). We also demonstrate that QG‐ 1 detection of GSH is feasible in a model protein system. QG‐ 1 was found to have extremely low cytotoxicity and was applied to determine the GSH concentration in live HeLa cells (5.40±0.87 m m ).