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BIBTEX RIS

Simultaneous recombinant 503 antigen recovery and endotoxin removal from E. coli M15 homogenate using expanded bed adsorption chromatography

2017; Taylor & Francis; Volume: 52; Issue: 11 Linguagem: Inglês

10.1080/01496395.2017.1305411

1520-5754

Francisco Canindé de Sousa Júnior, Vitor Troccoli Ribeiro, Abimaelle Silva Chibério, Laura Pires da Mata Costa, Carlos Eduardo de Araújo Padilha, Daniella Regina Arantes Martins, Gorete Ribeiro de Macêdo, Everaldo Silvino dos Santos,

Antimicrobial Peptides and Activities

This study evaluated the simultaneous recovery of recombinant 503 antigen and endotoxin removal from E. coli homogenate by immobilized metal affinity- expanded bed adsorption chromatography (EBAC), using the non-ionic surfactant Triton X-114 during the washing step. The strategy employed was able to remove more than 99% of endotoxin in all conditions tested, and the lowest residual concentration obtained was 10.22 EU/mL, with a recovery of 503 antigen ranging from 41.4% to 61.1%. Therefore, the procedure of integrating the clarification, recovery of protein, and endotoxin removal into a unit operation exploiting the EBAC was successful.