Generation of non-transgenic iPS cells from human cord blood CD34 + cells under animal component-free conditions
2017; Elsevier BV; Volume: 21; Linguagem: Inglês
10.1016/j.scr.2017.03.022
ISSN1876-7753
AutoresAlexandra Haase, Gudrun Göhring, Ulrich Martin,
Tópico(s)Animal Genetics and Reproduction
ResumoRecently, many hurdles and limitations for production of clinically applicable iPSC derivatives have been overcome. Transgene-free iPSCs can be efficiently derived from easily accessible cell sources such as blood. Here we describe the generation of transgene-free hiPS cells from cord blood derived CD34+ cells, reprogrammed using CytoTune™ Sendai reprogramming vectors. CD34+ cell isolation, cultivation, reprogramming and establishment of resulting hiPSC lines were performed under the exclusive usage of animal-derived component-free (ADCF) materials and components.
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