First Report of Colletotrichum gloeosporioides Causing Anthracnose on Loquat in Pakistan
2017; American Phytopathological Society; Volume: 101; Issue: 8 Linguagem: Inglês
10.1094/pdis-11-16-1551-pdn
ISSN1943-7692
AutoresFarah Naz, Muhammad Fahim Abbas, C. A. Rauf, Aliya Tariq, Abdul Samad Mumtaz, Gulshan Irshad, Farid Asif Shaheen, Hassan Imran,
Tópico(s)Mycorrhizal Fungi and Plant Interactions
ResumoHomePlant DiseaseVol. 101, No. 8First Report of Colletotrichum gloeosporioides Causing Anthracnose on Loquat in Pakistan PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Colletotrichum gloeosporioides Causing Anthracnose on Loquat in PakistanF. Naz, M. F. Abbas, C. A. Rauf, A. Tariq, A. Mumtaz, G. Irshad, F. A. Shaheen, and I. HassanF. NazSearch for more papers by this author, M. F. AbbasSearch for more papers by this author, C. A. RaufSearch for more papers by this author, A. TariqSearch for more papers by this author, A. MumtazSearch for more papers by this author, G. IrshadSearch for more papers by this author, F. A. ShaheenSearch for more papers by this author, and I. HassanSearch for more papers by this authorAffiliationsAuthors and Affiliations F. Naz M. F. Abbas C. A. Rauf A. Tariq A. Mumtaz G. Irshad , Department of Plant Pathology, Pir Mehr Ali Shah-Arid Agriculture University Rawalpindi, Pakistan F. A. Shaheen , Department of Entomology, Pir Mehr Ali Shah-Arid Agriculture University Rawalpindi, Pakistan I. Hassan , Department of Horticulture, Pir Mehr Ali Shah-Arid Agriculture University Rawalpindi, Pakistan. Published Online:5 Jun 2017https://doi.org/10.1094/PDIS-11-16-1551-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Anthracnose is a destructive postharvest (Palou et al. 2016) and foliar disease of loquat (Eriobotria japonica). Anthracnose was observed in loquat orchards of Taxila (33°44′13″N. 72°47′57″E) and Wah Cantt (33°46′17″N, 72°45′3″E) areas of Pakistan during April 2013 to July 2015. Leaf symptoms initiated as small circular necrotic spots that enlarged into reddish brown, round to irregular necrotic brown areas visible on adaxial and abaxial surfaces. Fruit exhibited dark brown sunken lesions, which later turned into black, hard, and shriveled mummies. Anthracnose affected 39 and 23% of the plants in Taxila and Wah Cantt, respectively. Leaf (120) and fruit (80) samples were collected from 10 loquat orchards. Small pieces (5 to 10 mm2) were cut from the margins of lesions on fruits and leaves, surface disinfested with 1% sodium hypochlorite for 2 min, washed three times in sterilized distilled water, dried, and placed aseptically on Czapek Dox agar (CDA) plates (Thom and Raper 1945), followed by incubation at 25°C for 4 to 5 days. White cottony mycelia with a concentric zone of shiny orange conidial masses were consistently observed on CDA after 4 days. Conidia were hyaline, cylindrical with rounded ends, and aseptate, measuring 12.7 to 18.8 × 3.3 to 6.7 µm (mean 15.5 × 4.8 μm, n = 50 conidia). Appressoria were light brown, ovate to obovate, and 6 to 10 × 4.5 to 7.5 µm (mean 8.6 × 5.8 μm). All these characteristics matched with those described for Colletotrichum gloeosporioides (Sawant et al. 2012). For verification of pathogen identity, DNA of two representative isolates (TAX-1 and WAH-1) was extracted and three loci including the internal transcribed spacer (ITS) region, actin (ACT), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were amplified using primers ITS1/ITS4, ACT512F/ACT783R, and GDF/GDR, respectively (Weir et al. 2012). The purified PCR products were sequenced by Macrogen Inc., Korea. GenBank accession numbers for isolate TAX-1 (KR075160, KX185669, KX378683) and WAH-1 (KR075159, KX216403, KX379458) were assigned for ITS, ACT, and GAPDH, respectively. BLAST analyses showed 98 to 100% identity with C. gloeosporioides accessions HQ874906, KF712382, and HQ022565 for ITS, ACT, and GAPDH, respectively. In order to determine the pathogenicity of the isolates, conidial suspensions (1.5 × 105 conidia/ml) were prepared for each of 35 isolates, which were sprayed until run-off on three detached healthy, unwounded leaves and fruit for each isolate. Three control leaves and fruit were inoculated with sterile distilled water and placed at 25 ± 2°C. The symptoms on all inoculated leaves initiated as pinpoint, round, reddish brown, circular necrotic spots after 3 days that later coalesced into irregular spots. On fruit, dark brown necrotic sunken lesions developed after 4 days, which later covered the whole fruit. Symptoms were similar to those observed in the orchards, and control leaves and fruit remained symptomless. This is the first report of C. gloeosporioides infecting loquat in Pakistan. Further studies are needed to ascertain the best control measures for the management of the disease in commercial orchards.References:Palou, L., et al. 2016. Eur. J. Plant Pathol. 4:847. https://doi.org/10.1007/s10658-016-0964-0 Crossref, ISI, Google ScholarSawant, I. S., et al. 2012. Australas. Plant Pathol. 41:493. https://doi.org/10.1007/s13313-012-0143-5 Crossref, ISI, Google ScholarThom, C., and Raper, K. B. 1945. Manual of Aspergilli. Williams & Wilkins, Baltimore, MD. Crossref, Google ScholarWeir, B. S., et al. 2012. Stud. Mycol. 73:115. https://doi.org/10.3114/sim0011 Crossref, ISI, Google ScholarFinancial assistance received from Pakistan Science Foundation (project no. PSF/NSLP/P-UAAR 501) for this study is greatly acknowledged.DetailsFiguresLiterature CitedRelated Vol. 101, No. 8 August 2017SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 12 Jul 2017Published: 5 Jun 2017First Look: 27 Mar 2017Accepted: 14 Mar 2017 Pages: 1550-1550 Information© 2017 The American Phytopathological SocietyCited byGene expression, molecular docking, and molecular dynamics studies to identify potential antifungal compounds targeting virulence proteins/genes VelB and THR as possible drug targets against Curvularia lunata12 December 2022 | Frontiers in Molecular Biosciences, Vol. 9Identification and Observation of Infection Processes of Colletotrichum Species Associated with Pearl Plum Anthracnose in Guangxi, ChinaRong Huang, Qing Gui, Yujie Zhang, Wenxiu Sun, Lihua Tang, Suiping Huang, Tangxun Guo, Qili Li, Jianyou Mo, Huiye Huang, Mingzhong Fan, Zongbin Zhang, and Tom Hsiang20 November 2022 | Plant Disease, Vol. 106, No. 12The Regulatory Mechanisms and Control Technologies of Chilling Injury and Fungal Diseases of Postharvest Loquat Fruit12 December 2022 | Plants, Vol. 11, No. 24Glomerella cingulata (anthracnose)CABI Compendium, Vol. CABI CompendiumDiversity of fungal pathogens associated with loquat and development of novel virulence scales14 October 2021 | PLOS ONE, Vol. 16, No. 10Colletotrichum species and complexes: geographic distribution, host range and conservation status29 September 2021 | Fungal Diversity, Vol. 110, No. 1First report of leaf spot on Amorphophallus muelleri caused by Colletotrichum gloeosporioides in Yunnan, China6 December 2019 | Journal of Plant Pathology, Vol. 102, No. 2Colletotrichum eriobotryae sp. nov. and C. nymphaeae, the anthracnose pathogens of loquat fruit in central Taiwan, and their sensitivity to azoxystrobin11 March 2020 | Mycological Progress, Vol. 19, No. 4Identification of antifungal compounds from slender amaranth1 January 2020 | Planta Daninha, Vol. 38Phylogenetic and morphological identification of Colletotrichum godetiae, a novel pathogen causing anthracnose on loquat fruits (Eriobotrya japonica)31 August 2019 | Journal of Plant Diseases and Protection, Vol. 126, No. 6First Report of Anthracnose Caused by Colletotrichum nymphaeae on Loquat Fruit in ChinaW. X. Wu, Y. Liu, X. Q. Huang, and L. Zhang18 October 2017 | Plant Disease, Vol. 102, No. 1
Referência(s)