Anti-ACSA-2 defines a novel monoclonal antibody for prospective isolation of living neonatal and adult astrocytes
2017; Wiley; Volume: 65; Issue: 6 Linguagem: Inglês
10.1002/glia.23140
ISSN1098-1136
AutoresChristina Geraldine Kantzer, Camille Boutin, Ina D. Herzig, Carolina Wittwer, Sandy Reiß, Marie Catherine Tiveron, Jan Drewes, Thomas Dino Rockel, Stefanie Ohlig, Jovica Ninkovic, Harold Cremer, Sandra Pennartz, Melanie Jungblut, Andreas Bosio,
Tópico(s)Neonatal and fetal brain pathology
ResumoGliaVolume 65, Issue 6 p. 990-1004 RESEARCH ARTICLE Anti-ACSA-2 defines a novel monoclonal antibody for prospective isolation of living neonatal and adult astrocytes Christina G. Kantzer, Christina G. Kantzer Miltenyi Biotec GmbH, Friedrich-Ebert-Straße 68, Bergisch Gladbach, 51429 GermanySearch for more papers by this authorCamille Boutin, Camille Boutin Aix-Marseille Université, CNRS, Institut de Biologie du Développement de Marseille, Campus de Luminy, Marseille, 13288 FranceSearch for more papers by this authorIna D. Herzig, Ina D. Herzig Miltenyi Biotec GmbH, Friedrich-Ebert-Straße 68, Bergisch Gladbach, 51429 GermanySearch for more papers by this authorCarolina Wittwer, Carolina Wittwer Miltenyi Biotec GmbH, Friedrich-Ebert-Straße 68, Bergisch Gladbach, 51429 GermanySearch for more papers by this authorSandy Reiß, Sandy Reiß Miltenyi Biotec GmbH, Friedrich-Ebert-Straße 68, Bergisch Gladbach, 51429 GermanySearch for more papers by this authorMarie Catherine Tiveron, Marie Catherine Tiveron Aix-Marseille Université, CNRS, Institut de Biologie du Développement de Marseille, Campus de Luminy, Marseille, 13288 FranceSearch for more papers by this authorJan Drewes, Jan Drewes Miltenyi Biotec GmbH, Friedrich-Ebert-Straße 68, Bergisch Gladbach, 51429 GermanySearch for more papers by this authorThomas D. Rockel, Thomas D. Rockel Miltenyi Biotec GmbH, Friedrich-Ebert-Straße 68, Bergisch Gladbach, 51429 GermanySearch for more papers by this authorStefanie Ohlig, Stefanie Ohlig Institute of Stem Cell Research, Helmholtz Center Munich, German Research Center for Environmental Health, Großhadernerstr.9, Planegg/Munich, 82152 Germany Physiological Genomics, Biomedical Center, Ludwig-Maximilian University Munich, Großhadernerstr.9, Planegg/Munich, 82152 GermanySearch for more papers by this authorJovica Ninkovic, Jovica Ninkovic Institute of Stem Cell Research, Helmholtz Center Munich, German Research Center for Environmental Health, Großhadernerstr.9, Planegg/Munich, 82152 Germany Physiological Genomics, Biomedical Center, Ludwig-Maximilian University Munich, Großhadernerstr.9, Planegg/Munich, 82152 GermanySearch for more papers by this authorHarold Cremer, Harold Cremer Aix-Marseille Université, CNRS, Institut de Biologie du Développement de Marseille, Campus de Luminy, Marseille, 13288 FranceSearch for more papers by this authorSandra Pennartz, Sandra Pennartz Miltenyi Biotec GmbH, Friedrich-Ebert-Straße 68, Bergisch Gladbach, 51429 GermanySearch for more papers by this authorMelanie Jungblut, Melanie Jungblut Miltenyi Biotec GmbH, Friedrich-Ebert-Straße 68, Bergisch Gladbach, 51429 GermanySearch for more papers by this authorAndreas Bosio, Corresponding Author Andreas Bosio Andreas.Bosio@miltenyibiotec.de orcid.org/0000-0001-9776-2535 Miltenyi Biotec GmbH, Friedrich-Ebert-Straße 68, Bergisch Gladbach, 51429 GermanyCorrespondence Andreas Bosio, Miltenyi Biotec GmbH, 51429 Bergisch Gladbach, Germany. Email: Andreas.Bosio@miltenyibiotec.deSearch for more papers by this author Christina G. Kantzer, Christina G. Kantzer Miltenyi Biotec GmbH, Friedrich-Ebert-Straße 68, Bergisch Gladbach, 51429 GermanySearch for more papers by this authorCamille Boutin, Camille Boutin Aix-Marseille Université, CNRS, Institut de Biologie du Développement de Marseille, Campus de Luminy, Marseille, 13288 FranceSearch for more papers by this authorIna D. Herzig, Ina D. Herzig Miltenyi Biotec GmbH, Friedrich-Ebert-Straße 68, Bergisch Gladbach, 51429 GermanySearch for more papers by this authorCarolina Wittwer, Carolina Wittwer Miltenyi Biotec GmbH, Friedrich-Ebert-Straße 68, Bergisch Gladbach, 51429 GermanySearch for more papers by this authorSandy Reiß, Sandy Reiß Miltenyi Biotec GmbH, Friedrich-Ebert-Straße 68, Bergisch Gladbach, 51429 GermanySearch for more papers by this authorMarie Catherine Tiveron, Marie Catherine Tiveron Aix-Marseille Université, CNRS, Institut de Biologie du Développement de Marseille, Campus de Luminy, Marseille, 13288 FranceSearch for more papers by this authorJan Drewes, Jan Drewes Miltenyi Biotec GmbH, Friedrich-Ebert-Straße 68, Bergisch Gladbach, 51429 GermanySearch for more papers by this authorThomas D. Rockel, Thomas D. Rockel Miltenyi Biotec GmbH, Friedrich-Ebert-Straße 68, Bergisch Gladbach, 51429 GermanySearch for more papers by this authorStefanie Ohlig, Stefanie Ohlig Institute of Stem Cell Research, Helmholtz Center Munich, German Research Center for Environmental Health, Großhadernerstr.9, Planegg/Munich, 82152 Germany Physiological Genomics, Biomedical Center, Ludwig-Maximilian University Munich, Großhadernerstr.9, Planegg/Munich, 82152 GermanySearch for more papers by this authorJovica Ninkovic, Jovica Ninkovic Institute of Stem Cell Research, Helmholtz Center Munich, German Research Center for Environmental Health, Großhadernerstr.9, Planegg/Munich, 82152 Germany Physiological Genomics, Biomedical Center, Ludwig-Maximilian University Munich, Großhadernerstr.9, Planegg/Munich, 82152 GermanySearch for more papers by this authorHarold Cremer, Harold Cremer Aix-Marseille Université, CNRS, Institut de Biologie du Développement de Marseille, Campus de Luminy, Marseille, 13288 FranceSearch for more papers by this authorSandra Pennartz, Sandra Pennartz Miltenyi Biotec GmbH, Friedrich-Ebert-Straße 68, Bergisch Gladbach, 51429 GermanySearch for more papers by this authorMelanie Jungblut, Melanie Jungblut Miltenyi Biotec GmbH, Friedrich-Ebert-Straße 68, Bergisch Gladbach, 51429 GermanySearch for more papers by this authorAndreas Bosio, Corresponding Author Andreas Bosio Andreas.Bosio@miltenyibiotec.de orcid.org/0000-0001-9776-2535 Miltenyi Biotec GmbH, Friedrich-Ebert-Straße 68, Bergisch Gladbach, 51429 GermanyCorrespondence Andreas Bosio, Miltenyi Biotec GmbH, 51429 Bergisch Gladbach, Germany. Email: Andreas.Bosio@miltenyibiotec.deSearch for more papers by this author First published: 20 March 2017 https://doi.org/10.1002/glia.23140Citations: 48 Funding information: The work was supported by the EU seventh framework program DopaNew, Grant /Award Number: #286021. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract Astrocytes are the most abundant cell type of the central nervous system and cover a broad range of functionalities. We report here the generation of a novel monoclonal antibody, anti-astrocyte cell surface antigen-2 (Anti-ACSA-2). Flow cytometry, immunohistochemistry and immunocytochemistry revealed that Anti-ACSA-2 reacted specifically with a not yet identified glycosylated surface molecule of murine astrocytes at all developmental stages. It did not show any labeling of non-astroglial cells such as neurons, oligodendrocytes, NG2+ cells, microglia, endothelial cells, leukocytes, or erythrocytes. Co-labeling studies of GLAST and ACSA-2 showed largely overlapping expression. However, there were also notable differences in protein expression levels and frequencies of single-positive subpopulations of cells in some regions of the CNS such as cerebellum, most prominently at early postnatal stages. In the neurogenic niches, the dentate gyrus of the hippocampus and the subventricular zone (SVZ), again a general overlap with slight differences in expression levels were observed. ACSA-2 was unlike GLAST not sensitive to papain-based tissue dissociation and allowed for a highly effective, acute, specific, and prospective purification of viable astrocytes based on a new rapid sorting procedure using Anti-ACSA-2 directly coupled to superparamagnetic MicroBeads. In conclusion, ACSA-2 appears to be a new surface marker for astrocytes, radial glia, neural stem cells and bipotent glial progenitor cells which opens up the possibility of further dissecting the characteristics of astroglial subpopulations and lineages. Citing Literature Supporting Information Additional Supporting Information may be found in the online version of this article. Filename Description glia23140-sup-0001-suppfig1.tif7.9 MB Supporting Information Figure 1. glia23140-sup-0002-suppfig2.tif12.3 MB Supporting Information Figure 2. glia23140-sup-0003-suppfig3.tif203.7 KB Supporting Information Figure 3. glia23140-sup-0004-suppfig4.tif6.5 MB Supporting Information Figure 4. glia23140-sup-0005-suppfig5.tif3.6 MB Supporting Information Figure 5. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume65, Issue6June 2017Pages 990-1004 RelatedInformation
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