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Isolation of <i>Streptococcus gallolyticus</i> with very high degradability of condensed tannins from feces of the wild Japanese rock ptarmigans on Mt. Tateyama

2017; Microbiology Research Foundation; Volume: 63; Issue: 3 Linguagem: Inglês

10.2323/jgam.2016.09.003

1349-8037

Sayaka Tsuchida, Koichi Murata, Moriya Ohkuma, Kazunari Ushida,

Enzyme Production and Characterization

ing on the snow-covered Mt.Tateyama, were at least 80 times more active in tannase than those previously shown in the strain from koala, Phascolarctos cinereus, and at least 8 times more active than those in the brushtail possum, Trichosurus vulpecula.Freshly defecated cecal feces, or rectal feces, were promptly collected from the snow following close observation (ca. 5 m distance) of the birds on Mt.Tateyama at the end of November 2014 and the middle of May 2015.A portion of the feces was streaked on a BL plate (Nissui, Tokyo, Japan), which was supplemented with 5% defibrinated chicken blood, or a GAM plate (Nissui, Tokyo, Japan).Plates were immediately placed in a plastic bag containing an Anaeropouch (Mitsubishi Gas Chemical, Tokyo, Japan) and then incubated at 41∞C in a portable incubator after returning to the Raicyo-so Lodge (15-30 minute after collection), where electricity was available.After overnight culture, plates were placed in a styrene foam box with cooling devices and transported to our laboratory.Visible colonies were chosen and re-streaked on the same plate medium.After purification, partial 16S rRNA gene sequences were determined as described previously (Tsuchida et al., 2014).Similar sequences were retrieved from public databases, and calculations for pairwise sequence similarities for 16S rRNA genes were made using MEGA version 6.06 (Tamura et al., 2013).Multiple sequence alignments were performed using the CLUSTAL W program (Thompson et al., 1994) and phylogenetic trees were constructed using the neighbor-joining method (Saitou and Nei, 1987).Tree topology was evaluated by a bootstrap analysis with 1000 replicates using CLUSTAL W.In accordance with Osawa (1990), we prepared tannin-Isolation of Streptococcus gallolyticus with very high degradability of condensed tannins from feces of the wild Japanese rock ptarmigans on Mt.Tateyama