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Revisado por pares
First Worldwide Report of Colletotrichum pseudoacutatum Causing Anthracnose on Syzygium jambos
2017; American Phytopathological Society; Volume: 101; Issue: 7 Linguagem: Inglês
10.1094/pdis-02-17-0261-pdn
ISSN1943-7692
AutoresWilliam Rosa de Oliveira Soares, Ana Clara Ribeiro Quitania, Robert G. Miller, Adalberto C. Café‐Filho, Ailton Reis, Danilo Batista Pinho,
Tópico(s)Yeasts and Rust Fungi Studies
ResumoHomePlant DiseaseVol. 101, No. 7First Worldwide Report of Colletotrichum pseudoacutatum Causing Anthracnose on Syzygium jambos PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Worldwide Report of Colletotrichum pseudoacutatum Causing Anthracnose on Syzygium jambosW. R. O. Soares, A. C. R. Quitania, R. N. G. Miller, A. C. Café-Filho, A. Reis, and D. B. PinhoW. R. O. SoaresSearch for more papers by this author, A. C. R. QuitaniaSearch for more papers by this author, R. N. G. MillerSearch for more papers by this author, A. C. Café-FilhoSearch for more papers by this author, A. ReisSearch for more papers by this author, and D. B. PinhoSearch for more papers by this authorAffiliationsAuthors and Affiliations W. R. O. Soares A. C. R. Quitania R. N. G. Miller A. C. Café-Filho , Departamento de Fitopatologia, Universidade de Brasília, Distrito Federal, Brazil, 70910-900 A. Reis , Empresa Brasileira de Pesquisa Agropecuária, Centro Nacional de Pesquisa de Hortaliças, Brasília, DF, Brazil, 70351-970 D. B. Pinho , Departamento de Fitopatologia, Universidade de Brasília, Distrito Federal, Brazil 70910-900. Published Online:9 May 2017https://doi.org/10.1094/PDIS-02-17-0261-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Colletotrichum species are usually well characterized on commercial fruit, but understudied on exotic fruits. Syzygium jambos (Myrtaceae) is an ornamental and fruit tree from Southeast Asia that is now widespread in Brazil (Lorenzi et al. 2006). In 2016, S. jambos fruits with anthracnose symptoms were observed distributed randomly on several individual trees on the campus of the Universidade de Brasília, in Brasília, central Brazil. Initial symptoms appeared as circular, light brown lesions that later became dark brown and sunken with orange spore masses. A fungus was isolated from symptomatic fruits and single-spore cultures were obtained following plating on potato dextrose agar (PDA). Conidia were hyaline, aseptate, straight, cylindrical to fusiform with both ends acute, and 11 to 18.5 µm long and 3.5 to 5 µm wide. Conidia with acute ends are typical of the Colletotrichum acutatum complex (Damm et al. 2012). The monosporic culture was deposited at the Coleção de Culturas Fúngicas da Universidade de Brasília (code CCUB 272) and used for molecular characterization. Partial sequences of the glyceraldehyde-3-phosphate dehydrogenase gene (GAPDH), β-tubulin 2 gene (TUB2), and ribosomal DNA internal transcribed spacer region (ITS) (accession nos. KY344753 to KY344755) were employed in a search against the Q-bank fungal database, with C. pseudoacutatum isolate CBS 436.77 retrieved with 100% identities. In order to confirm the pathogenicity of this fungus, 5 mm mycelial disks or conidial suspensions (105 conidia/ml) obtained from a 7-day-old culture (grown at 25°C) were deposited directly onto either intact S. jambos fruit surfaces or surfaces superficially wounded using a sterilized needle. PDA discs or sterilized distilled water were used on control fruits and treatments were repeated five times. After inoculation, fruits were placed in a tray, covered with transparent plastic film, and incubated at 25°C. Symptoms were observed on all fruits 3 days after inoculation with fungal material, while mock-inoculated control fruits remained healthy. Fungal cultures reisolated from lesions exhibited morphological characteristics identical the isolate originally inoculated and to C. pseudoacutatum Damm, P.F. Cannon & Crous. C. pseudoacutatum is a species with acute-ended conidia that is phylogenetically distinct from the C. acutatum complex. To date, only one isolate of this species has been reported, on seedlings of Pinus radiata in Chile (Damm et al. 2012). Only two Colletotrichum records have been previously observed on S. jambos, in disease indexes, with C. gloeosporioides reported in the United States of America (Alfieri et al. 1984) and Glomerella cingulata in Hong Kong (Lu et al. 2000). Without ruling out the possibility that there are more Colletotrichum species associated to jambo anthracnose, this is the first unequivocal identification of C. pseudoacutatum causing anthracnose on S. jambos fruits in Brazil or worldwide.References:Alfieri, S. A., Jr., et al. 1984. Ind. Bull. 11:1. Google ScholarDamm, U., et al. 2012. Stud. Mycol. 73:37. https://doi.org/10.3114/sim0010 Crossref, ISI, Google ScholarLorenzi, H., et al. 2006. Frutas brasileiras e exóticas cultivadas. Editora Plantarum, São Paulo, Brazil. Google ScholarLu, B., et al. 2000. Checklist of Hong Kong Fungi. Fungal Diversity Press, Hong Kong. Google ScholarDetailsFiguresLiterature CitedRelated Vol. 101, No. 7 July 2017SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 20 Jun 2017Published: 9 May 2017First Look: 3 Apr 2017Accepted: 28 Mar 2017 Pages: 1322-1322 Information© 2017 The American Phytopathological SocietyCited byColletotrichum pseudoacutatumCABI Compendium, Vol. CABI CompendiumColletotrichum species and complexes: geographic distribution, host range and conservation status29 September 2021 | Fungal Diversity, Vol. 110, No. 1
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