First Report of Kalanchoe mosaic virus and Kalanchoe latent virus Infecting Ghost Plant ( Graptopetalum paraguayense ) in Italy
2017; American Phytopathological Society; Volume: 101; Issue: 8 Linguagem: Inglês
10.1094/pdis-03-17-0338-pdn
ISSN1943-7692
AutoresR. Sorrentino, Armelle Marais, Christophe Fauré, Sébastien Theil, D. Alioto, Thierry Candresse,
Tópico(s)Plant-Microbe Interactions and Immunity
ResumoHomePlant DiseaseVol. 101, No. 8First Report of Kalanchoe mosaic virus and Kalanchoe latent virus Infecting Ghost Plant (Graptopetalum paraguayense) in Italy PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Kalanchoe mosaic virus and Kalanchoe latent virus Infecting Ghost Plant (Graptopetalum paraguayense) in ItalyR. Sorrentino, A. Marais, C. Faure, S. Theil, D. Alioto, and T. CandresseR. Sorrentino, A. Marais, C. Faure, S. Theil, D. Alioto, and T. CandresseAffiliationsAuthors and Affiliations R. Sorrentino , Università degli Studi di Napoli “Federico II”, Biology and Protection of Agricultural and Forest Systems (BIPAF) - Dipartimento di Agraria, 80055 Portici, Italy A. Marais C. Faure S. Theil , UMR 1332 Biologie du Fruit et Pathologie, INRA, Univ. Bordeaux, CS 20032, 33882 Villenave d’Ornon Cedex, France D. Alioto , Università degli Studi di Napoli “Federico II”, Biology and Protection of Agricultural and Forest Systems (BIPAF) - Dipartimento di Agraria, 80055 Portici, Italy T. Candresse , UMR 1332 Biologie du Fruit et Pathologie, INRA, Univ. Bordeaux, CS 20032, 33882 Villenave d’Ornon Cedex, France. Published Online:12 May 2017https://doi.org/10.1094/PDIS-03-17-0338-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat In spring 2013 during a survey for plant quarantine pests, a symptomatic ornamental succulent plant (Graptopetalum paraguayense W., ghost plant) was identified in a private garden in the Campania region (southern Italy). The plant showed symptoms consisting in greenish to dark green spots visible both on old and young leaves and a mild speckling present mostly on the older leaves. The symptoms were less visible on the thicker leaves. A panel of indicator plants including Chenopodium quinoa, C. amaranticolor, Cucumis sativus, Datura metel, D. stramonium, Nicotiana benthamiana, N. glutinosa, N. rustica, N. tabacum cv. White Burley, and Vigna unguiculata, was sap-inoculated and only C. amaranticolor plants developed symptoms, in the form of pinpoint chlorotic local lesions, 7 to 10 days after the inoculation. The lesion centers became necrotic and surrounded by mildly chlorotic tissue, 20 days post inoculation. In several attempts, sap transmission from these local lesions to healthy C. amaranticolor plants resulted in an increased number of lesions. To better understand the etiology of these symptoms, the succulent plant was analyzed in the frame of a metagenomic experiment. Double-stranded RNAs were extracted from symptomatic leaves, as previously reported (Candresse et al. 2013), and a cDNA library generated from these dsRNAs sequenced using the Illumina Miseq next-generation sequencing platform. A de novo assembly of the reads generated contigs that were further assembled into two scaffolds of respectively 8.3 kb and 9.2 kb; BLASTn analyses of the scaffolds showed 96 and 84% identity with the deposited sequences of Kalanchoe latent virus (KLV, fam. Betaflexiviridae, gen. Carlavirus; FJ531634) and Kalanchoe mosaic virus (KMV, fam. Potyviridae, gen. Potyvirus; GQ497732), respectively. In both cases, these values clearly fall within the accepted species variability boundaries. The NGS data allowed us to obtain almost the complete genomes for both agents, with only small regions missing for both viruses. The sequences were completed, and the genome ends were determined by 5′ and 3′ RACE using the SMARTer RACE 5′/3′ Kit (Clontech). The complete sequences have been deposited in GenBank with the accession numbers KY385304 (KLV) and KY385303 (KMV). In order to confirm the presence of both agents in the initial succulent plant, RT-PCR experiments were carried out using total nucleic acids and two different primer pairs: a specific primer pair for the detection of the carlavirus (KLV-fw 5′-TGGTTATCATGTGCGCTAGTGTG-3′; KLV-rev 5′-CTTTCTTCCAGGATTTAGTCTCAC-3′) and the Polypoty primer pair NibF2/NibR3 (Zheng et al. 2008) for the potyvirus. The sequences of the expected size PCR products showed 100% identity with the NGS data for both viruses. Kalanchoe blossfeldiana has been reported as host of KMV in Denmark (Husted et al. 1994) and of KLV in the U.S.A. (Hearon 1982) and to our knowledge, this is the first report of a mixed infection in G. paraguayense by these two agents in Italy or elsewhere in the world, extending the host range of these two viruses to another species in the Crassulaceae family. The obtained sequence also represents the first full-length KMV sequence. The potential contribution to the symptoms observed on the G. paraguayense plant as well as the distribution of these two agents on this or other succulent hosts in the world remain to be investigated.References:Candresse, T., et al. 2013. Phytopathology 103:293. https://doi.org/10.1094/PHYTO-10-12-0275-R Link, ISI, Google ScholarHearon, S. S. 1982. Phytopathology 72:838. https://doi.org/10.1094/Phyto-71-645 Crossref, ISI, Google ScholarHusted, K., et al. 1994. Phytopathology 84:161. https://doi.org/10.1094/Phyto-84-161 Crossref, ISI, Google ScholarZheng, L., et al. 2008. PLoS One 3:e1586. https://doi.org/10.1371/journal.pone.0001586 Crossref, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 101, No. 8 August 2017SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 12 Jul 2017Published: 12 May 2017First Look: 11 Apr 2017Accepted: 4 Apr 2017 Pages: 1560-1560 Information© 2017 The American Phytopathological SocietyCited byKalanchoe latent virusCABI Compendium, Vol. CABI CompendiumKalanchoe mosaic virusCABI Compendium, Vol. CABI CompendiumGraptopetalum paraguayense (Ghost plant)6 June 2020Kalanchoe blossfeldiana (Kalanchoe)6 June 2020
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