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Photoprotective, antibacterial activity and determination of phenolic compounds of Neoglaziovia variegata (Bromeliaceae) by high performance liquid chromatography-diode array detector (HPLC-DAD) analysis

2015; Academic Journals; Volume: 9; Issue: 22 Linguagem: Inglês

10.5897/ajpp2015.4315

1996-0816

Gonçalves de Oliveira Júnior Raimundo, Rocha Souza Grasielly, Leite Guimaraes Amanda, Paula de Oliveira Ana, de Souza Araujo Camila, Cabral Silva Juliane, Gomes Marques Pacheco Alessandra, Raquel Gomes de Lima Saraiva Sarah, Araújo Rolim Larissa, Jose Rolim Neto Pedro, Nora Castro Rosane, Jackson Roberto Guedes da Silva Almeida,

Fern and Epiphyte Biology

The photoprotective activity of flowers and antibacterial activity of the leaves of Neoglaziovia variegata were investigated. This study also identified the first phenolic compounds of the extracts and fractions of this species by high performance liquid chromatography (HPLC). The photoprotective activity was measured using the spectrophotometric method. The antibacterial activity was evaluated by microdilution method. The chromatographic analysis was performed in HPLC coupled with iode array detector (DAD) detector, using wavelengths of 254 and 320 nm. Nv-Fl-EtOH, Nv-Fl-CHCl3 and Nv-Fl-AcOEt showed characteristic absorption bands in regions UVB and UVA in a concentration-dependent manner. Nv-Fl-CHCl3 presented the highest sun protection factor (11.45 ± 2.87). Extracts from the leaves showed activity against most of the microorganisms tested, especially Bacillus cereus, Escherichia coli, Salmonella enterica, Serratia marcescens and Shigella flexneri. HPLC analysis revealed the presence of six phenolics: two flavonoids (isoquercetin and kaempferol-3-O­-rhamnoside) and four phenolic acids (caffeic, protocatechuic, p-coumaric and vanillic acids). This result justifies the biological properties, that the species has demonstrated and can lead other phytochemical studies aiming the isolation of these compounds. Key words: Bromeliaceae, Neoglaziovia variegata, photoprotective activity, antibacterial activity, phenolic compounds.