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Development of a Biosensor Modified with Nanoparticles for Sensitive Detection of Gluten by Chronoamperometry

2017; Institute of Physics; Volume: 76; Issue: 1 Linguagem: Inglês

10.1149/07601.0103ecst

2151-2051

Guadalupe Ontiveros, Prisciliano Hernández, Israel S. Ibarra, Julia Maria Dominguez, José A. Rodrı́guez,

Advanced biosensing and bioanalysis techniques

A novel and sensitive biosensor electrochemical was designed and developed and applied in the gluten detection bychronoamperometry in food samples. Protease enzyme was electrodeposited on carbon electrode modified with nanoparticles offullerene increasing the selectivity and surfacearea of electrode. Variables involved in the electrochemical system were optimized by a univariate design.Optimal conditions were: pH solution of 7.0, potential -0.7 V,enzyme immobilized (U) of 0.6.Under optimal conditions of analysis was obtained a linear range from 1.7-8.3 mg L-1, with a limit of detection of 0.54 mg L-1, repeatability and reproducibility was evaluated at two spiked levels (5.0 and 7.0 mg L-1)with relative standard deviation less than 10% in all cases. The proposed method was applied to analyze gluten in food samples based in cereals grains. The biosensor offers advantages in terms of simplicity, low cost, short analysis time, making it an alternative for analysis of gluten in matrix complex.