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Alpha-bisabolol Promotes Glioma Cell Death by Modulating the Adenosinergic System

2017; International Institute of Anticancer Research (IIAR) Conferences 1997. Athens, Greece. Abstracts; Volume: 37; Issue: 4 Linguagem: Inglês

10.21873/anticanres.11516

1791-7530

Franciane Brackmann Mendes, Letícia Scussel Bergamin, Cristiane dos Santos Stuepp, Elizandra Braganhol, Thatiana Ferreira Terroso, Adriana Raffin Pohlmann, Sı́lvia Stanisçuaski Guterres, Ana Maria Oliveira Battastini,

Cancer, Lipids, and Metabolism

Background/Aim: Glioblastoma multiforme is the most malignant type of glioma. Alpha-bisabolol is an essential oil reported as a potent cell death agent. In the present work, we evaluated the effect of alpha-bisabolol on ecto-5'-nucleotidase/CD73, the most well-characterized enzymatic source of adenosine, present in lipid rafts. Materials and Methods: Glioma cells were treated with alpha-bisabolol and, in some experiments, pre-treated with an A 3 antagonist. MTT assay (viability), malachite green method (ecto-5'-nucleotidase/CD73 activity) and quantitative polymerase chain reaction (qPCR) (A 3 mRNA) were carried out. Results: Alpha-bisabolol led to a decrease in C6 and U138-MG glioma cells viability, accompanied by an increase in ecto-5'-NT/CD73 activity. Pre-treatment with an A 3 antagonist reverted the effect of α-bisabolol with an increase of mRNA expression of this receptor. Conclusion: Our data indicated the participation of ecto-5'-nucleotidase/CD73 and A 3 receptor in the anti-proliferative effect of α-bisabolol on glioma cells.