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Normal Exfoliative Cytology of the Oral Cavity

1959; Volume: 71; Issue: 9-2 Linguagem: Inglês

10.4044/joma1947.71.9-2_6089

1882-4528

Toshiyuki Matsui,

Oral Health Pathology and Treatment

This study was made for the purpose of establishing the criteria of normal exfoliative cytology of the oral cavity. Clinically healthy twenty male adults with normal well-cared-for mouth were used and their smears were prepared from four areas of the oral mucosa-hard palate, gingiva, radix linguae and buccal mucosa, and stained by Papanicolaou and Giemsa stainings. And the distribution of keratinized cells was examined statistically. Concurrently histclogic sections were taken by gingivectomy and the specimens were staind by hematoxylineosin, azan, and Papanicolaou technics whose findings were compared with each other.The results obtained are as follows:1) By means of Papanicolaou technic cytoplasm of the epithelial cells showed different colors---non-keratinized blue, mixed with red or red mixed with blue, red and highly keratinized orange.2) Sizes of nucleus and cytoplasm showed somewhat difference according to the areas where smears were taken.3) Keratinization of the epithelial cells are highest at the hard palate, then gingiva and radix linguae are followed in the order. Almost no keratinized cells are noticed at the buccal mucosa.4) Neither parabasal cells nor atypical cells could be found in normal cases.5) White blood cell count showed a gradual decrease as epithelial cells showed the progress of keratinization.6) Nuclear-cytoplasmic ratio varied to some extent according to the localization. Nuclei of the epithelial cells of hard palate and gnigiva showed a higher percentage in their disappearance.7) Both keratinization grade and structural density were higher in the superficial layer and lower in the deeper layer in normal gingival epithelium.8) Minute strucutures of the cytoplasm and nuclei could be noticed more remarkably in Papanicolaou staining than in Giemsa staining.9) Almost same agreement could be found between exfoliative cytologic and histologic findings.