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Antimicrobial Activities and Cytotoxicity of Sisymbrium irio L Extract against Multi-Drug Resistant Bacteria (MDRB) and Candida albicans

2017; Excellent Publishers; Volume: 6; Issue: 4 Linguagem: Inglês

10.20546/ijcmas.2017.604.001

2319-7706

Gamal M. El Sherbiny, Saad A. Moghannem, Mohamed H. Sharaf,

Ethnobotanical and Medicinal Plants Studies

The present study was conducted to evaluate antimicrobial and cytotoxicactivity of Sisymbrium irio L extract against MDRB (Staphylococcus aureus, Enterococcus faecium, Klebsiella pneumoniae, Acinetobacter baumanni, Enterobacter cloacae and Pseudomonas aeruginosa) and Candida albicans. Antibiotic sensitivity profile was performed using disc diffusion method. Cytotoxicity was measured against African Green Monkey Kidney (VERO) cell line using the colorimetric MTT assay. Antimicrobial activity of aqueous Sisymbrium irio L extract showed inhibition activity against tested organisms Staphylococcus aureus (17mm), Enterococcus faecium (22mm), Klebsiella pneumoniae (15mm), Acinetobacter baumanni (18mm), Enterobacter cloacae (17mm), Pseudomonas aeruginosa (15mm), and Candida albicans (21mm). The highest activity was observed against Enterococcus faecium. This inhibition activity was higher than most of antibiotics used in the study. The crude extract was purified using column chromatography and visualized under UV using Thin Layer Chromatography (TLC). The minimum inhibitory concentration (MIC) values of purified active compound ranged from 31.25 to 125μg/ml while the minimum bactericidal concentration (MBC)was two-fold higher than MIC ranged from 62.5 to 250μg/ml. Cytotoxic activity of purified active compound showed little toxicity withIC50 exceeding400μg/ml after 24hr of incubation. The purified compound was identified using UV, IR, 1HNMR and mass spectroscopy. The analysis of data obtained indicated that it belongs to cyclo hexanone group.