Portal de Periódicos da CAPES

First Report of Wilt of Sugar Beet Caused by Gibellulopsis nigrescens in the Xinjiang Region of China

2017; American Phytopathological Society; Volume: 101; Issue: 7 Linguagem: Inglês

10.1094/pdis-01-17-0087-pdn

1943-7692

Yang Zhou, Z. Q. Zhao, Qingyuan Guo, Bin Lei,

Yeasts and Rust Fungi Studies

HomePlant DiseaseVol. 101, No. 7First Report of Wilt of Sugar Beet Caused by Gibellulopsis nigrescens in the Xinjiang Region of China PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Wilt of Sugar Beet Caused by Gibellulopsis nigrescens in the Xinjiang Region of ChinaY. Zhou, Z. Q. Zhao, Q. Y. Guo, and B. LeiY. Zhou, Z. Q. Zhao, Q. Y. Guo, and B. LeiAffiliationsAuthors and Affiliations Y. Zhou Z. Q. Zhao Q. Y. Guo , Laboratory of Plant Pathology, Department of Plant Protection, College of Agricultural, Xinjiang Agricultural University, Urumqi, XinJiang 830052, China B. Lei , Research Institute of Nuclear and Biotechnology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China. Published Online:9 May 2017https://doi.org/10.1094/PDIS-01-17-0087-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Sugar beet (Beta vulgaris L.) is a widely planted sugar crop worldwide. In 2014 and 2015, sugar beet plants (variety KWS0143) with wilt symptoms were observed in the Xinjiang Region of China. In August 2015, sugar beet plants with vein-delimited yellowing on leaves and light-brown vascular discoloration of petioles were collected from Yanqi, Qitai, and Yining in the Xinjiang Region. As the symptoms progressed, the leaf surface became covered by black mold. These symptomatic plants were sporadically distributed in fields. Petioles with typical symptoms were selected and washed with stile water, dipped in 95% ethanol, and dried. Petiole slices (4 mm3) with light-brown vascular tissue were sterilized in 1% sodium hypochlorite for 1 min and placed on potato dextrose agar (PDA) at 25°C for 5 days. Five Gibellulopsis nigrescens (Pehtybr.) Zare, Gams, and Summerbell (syn. Verticillium nigrescens) isolates were obtained on the PDA. Mycelia in the cultures were initially white but masses of melanized chlamydospores led to a black appearance in the center of the colony. Conidiophores were short, hyaline, and verticillately branched with three to four phialides in each whorl. Conidia were hyaline elliptical, straight, measuring from 3.5 to 9.5 × 2.5 to 3.5 μm (5 × 2.8 μm avg.). Morphological characteristics were similar to those described by Zare et al. (2007). To confirm the identity of the fungus, the complete internal transcribed spacer (ITS) region of rDNA of the pathogen was amplified with the primers ITS1/ITS4 and sequenced, with a resulting amplicon size of 535 bp. BLAST analysis of the amplicon sequence had 99% sequence identity with G. nigrescens (GenBank Accession Nos. AY555959 and HE972037). Pathogenicity tests were carried out using the toothpick inoculation technique. Prior to use, toothpicks were autoclaved twice in deionized water. Toothpicks colonized by the G. nigrescens strains (five strains evaluated individually) were used to stab-inoculate the base of petioles on healthy sugar beet plants (60-days-old, 10 to 12 leaves); control plants were only stabbed with sterile toothpicks. Plants were kept in a growth chamber at 20°C with a 12-h photoperiod. After 15 days, inoculated plants developed symptoms similar to diseased plants in fields. The symptoms included wilting with vein delimited yellowing and inward curling of the leaves. After 25 days, the symptomatic leaves died, while the control plants remained symptomless. In isolations from infested plants, G. nigrescens was reisolated, but no fungus was found in the control plants. On the basis of morphological and ITS sequence information, the fungus was identified as G. nigrescens. In sugar beet, wilt has been caused by Verticillium dahliae Kleb. in areas such as Germany, Greece, former Czechoslovakia, Netherlands, Spain, and Sweden, as well as Colorado, Idaho, Minnesota, Nebraska, North Dakota, and Washington in the United States (Brantner et al. 2008; Gaskill and Krentzer 1940; Karadimos et al. 2000,; Strausbaugh et al. 2016). However, wilt of sugar beet has also been caused by G. nigrescens in Canada (Zare et al. 2007) and, now for the first time, in the Xinjiang region of China.References:Brantner, J. R., et al. 2008. Plant Health Prog. https://doi.org/10.1094/PHP-2008-1212-01-BR. Google ScholarGaskill, J. O., and Krentzer, W. A. 1940. Phytopathology 30:769. Google ScholarKaradimos, D. A., et al. 2000. Plant Dis. 84:593. https://doi.org/10.1094/PDIS.2000.84.5.593C Link, Google ScholarStrausbaugh, C. A., et al. 2016. Can. J. Plant Pathol. 38:492. https://doi.org/10.1080/07060661.2016.1260639. Crossref, ISI, Google ScholarZare, R., et al. 2007. Nova Hedwigia 85:463. https://doi.org/10.1127/0029-5035/2007/0085-0463 Crossref, ISI, Google ScholarY. Zhou and Z. Q. Zhao contributed equally to this work.This study was financially supported by the demonstration base of Xinjiang Agricultural University produce-learn-research projects united training graduate.DetailsFiguresLiterature CitedRelated Vol. 101, No. 7 July 2017SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 20 Jun 2017Published: 9 May 2017First Look: 11 Apr 2017Accepted: 2 Apr 2017 Page: 1318 Information© 2017 The American Phytopathological SocietyCited byAnalysis of Soil Fungal Community in Aged Apple Orchards in Luochuan County, Shaanxi Province25 December 2022 | Agriculture, Vol. 13, No. 1Diversity analysis of leaf endophytic fungi and rhizosphere soil fungi of Korean Epimedium at different growth stages21 October 2022 | Environmental Microbiome, Vol. 17, No. 1Dark side of a bio-based and biodegradable plastic? Assessment of pathogenic microbes associated with poly(butylene succinate-co-adipate) under ambient and future climates using next-generation sequencing13 October 2022 | Frontiers in Plant Science, Vol. 13Co-composting of fresh tobacco leaves and soil: an exploration on the utilization of fresh tobacco waste in farmland5 September 2021 | Environmental Science and Pollution Research, Vol. 29, No. 6Changes in microbial community and enzyme activity in soil under continuous pepper cropping in response to Trichoderma hamatum MHT1134 application3 November 2021 | Scientific Reports, Vol. 11, No. 1Sugarcane monoculture drives microbial community composition, activity and abundance of agricultural-related microorganisms26 April 2021 | Environmental Science and Pollution Research, Vol. 28, No. 35Study on the Diversity of Fungal and Bacterial Communities in Continuous Cropping Fields of Chinese Chives (Allium tuberosum)BioMed Research International, Vol. 2020New plectosphaerellaceous species from Dutch garden soil14 September 2019 | Mycological Progress, Vol. 18, No. 9Changes in fungal community and diversity in strawberry rhizosphere soil after 12 years in the greenhouseJournal of Integrative Agriculture, Vol. 18, No. 3Four New Records of Ascomycete Species from Korea17 December 2018 | Mycobiology, Vol. 46, No. 4