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In vitro growth inhibition and cytotoxicity of Euphorbia caducifolia against four human cancer cell lines and its phytochemical characterisation

2017; Taylor & Francis; Volume: 31; Issue: 24 Linguagem: Inglês

10.1080/14786419.2017.1305380

1478-6427

Shaista Bano, Bina S. Siddiqui, Ahsana Dar, Sabira Begum, Faheema Siddiqui, Muhammad Kashif, Mudassar Azhar,

Phytochemistry and Bioactive Compounds

Several Euphorbia species have been used in folklore as cancer remedies, however, scientific studies on the cytotoxicity (in vitro studies) of Euphorbia caducifolia are lacking. In present study, anticancer potential of E. caducifolia aerial parts ethanol extract and its fractions were evaluated against human lung (NCI-H460), breast (MCF-7), prostate (PC-3) and cervical (HeLa) cancer cell lines, using sulphorhodamine-B in vitro cytotoxicity (in vitro studies) assay. The ethanol extract demonstrated growth inhibitory effect against all aforementioned cancer cell lines with IC50, 19–135 μg/mL and LC50, ~220 μg/mL, and its petroleum ether fraction obtained on bioactivity guided fraction showed highest activity with IC50, 28–70 μg/mL and LC50, 71 μg/mL against NCI-H460 and MCF-7 cell lines. Its phytochemicals were analysed by gas chromatography–mass spectrometry (GC-MS). The present study provides scientific justification for its traditional use against cancer.