A fully integrated, three-dimensional fluorescence to electron microscopy correlative workflow
2017; Elsevier BV; Linguagem: Inglês
10.1016/bs.mcb.2017.03.008
ISSN0091-679X
AutoresClàudia López, Cédric Bouchet‐Marquis, Christopher P. Arthur, Jessica L. Riesterer, Gregor Heiss, Guillaume Thibault, Lee Pullan, Sunjong Kwon, Joe W. Gray,
Tópico(s)Advanced Fluorescence Microscopy Techniques
ResumoWhile fluorescence microscopy provides tools for highly specific labeling and sensitive detection, its resolution limit and lack of general contrast has hindered studies of cellular structure and protein localization. Recent advances in correlative light and electron microscopy (CLEM), including the fully integrated CLEM workflow instrument, the FEI CorrSight with MAPS, have allowed for a more reliable, reproducible, and quicker approach to correlate three-dimensional time-lapse confocal fluorescence data, with three-dimensional focused ion beam–scanning electron microscopy data. Here we demonstrate the entire integrated CLEM workflow using fluorescently tagged MCF7 breast cancer cells.
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