Produção Nacional
Revisado por pares
Influência da estação do ano sobre as proteínas do plasma seminal de touros Limousin
2007; EDUFU; Volume: 13; Issue: 1 Linguagem: Inglês
ISSN
1983-0777
AutoresMarcelo George Mungai Chacur, Nelson Barbosa Machado Neto,
Tópico(s)Reproductive Physiology in Livestock
ResumoThe objective of this work was evaluating the season year influence upon seminal plasma proteins in Limousin bulls. Semen samples of twelve animals with 2-years-old were collected with electroejaculation, during winter, at 7 days intervals in a total of 55 samples. In summer, nine of these bulls were re-evaluated through the andrology tests. Samples of semen were centrifuged at 1500 g / 15 min and conditioned in eppendorf tubes and stored at -20oC until further processing. The Proteins were extracted from 200 μL of each sample in 2 mL of extraction buffer composed by 0.625 M Tris-HCl; pH 6.8, 2% SDS, 5% β-mercaptoethanol and 20% of glycerol. The protein was quantified and the electrophoresis was performed; the gels were fixed, and stained in the same solution with 2% of Coomassie Blue R250. In Bulls A, C, D, E and I the absence high molecular weight (HMW 55 KDa, 66 KDa and 80 KDa) proteins was verified in the winter and the one which suppose to be of low fertility (LF 40 KDa) absent in bull D, in summer. The bulls F, G e J showed presence of HMW (55KDa) in the winter. In bulls H, I and J HMW (55 KDa, 66 KDa or 80 KDa) proteins were present with a satisfactory semen condition. The bull I showed presence of HMW proteins (66 KDa and 80 KDa) in summer. It suggests that different seasons of the year may influence the presence or absence of proteins in seminal plasma.
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