Portal de Periódicos da CAPES

Artigo Produção Nacional Revisado por pares

BIBTEX RIS

Comparison of cytotoxicity, genotoxicity and immunological inflammatory biomarker activity of several endodontic sealers against immortalized human pulp cells

2017; Wiley; Volume: 51; Issue: 1 Linguagem: Inglês

10.1111/iej.12785

1365-2591

Frederico C. Martinho, Samira Esteves Afonso Camargo, Aletéia Massula Fernandes, Márcia Sampaio Campos, Renata Falchete do Prado, Carlos Henrique Camargo, Márcia Carneiro Valera,

Endodontics and Root Canal Treatments

Abstract Aim To establish an SV 40 T‐Ag‐transfected cell line of human pulp‐derived cells in order to compare the cytotoxicity, genotoxicity and to investigate the activities of immunological biomarkers of several endodontic sealers. Methodology Primary human pulp cells and transfected cells were cultured. Cell morphology and proliferation were analysed, and the expression of cell‐specific gene transcripts and proteins was detected by RT – PCR and immunohistochemistry. Transfection of human pulp‐derived cells resulted in an immortalized cell line retaining phenotypic characteristics from the primarily cells tested. The SV 40 T‐Ag‐transfected cells were cultured and stimulated by sealers (Apexit Plus, Real Seal, AH Plus, and Endo REZ ) to evaluate the cytotoxicity and genotoxicity by MTT and MTN assays, respectively. Immunological inflammatory biomarkers ( IL 6, IL 8 and TNF ‐α) were determined by ELISA assay. The differences between median values were statistically analysed using Kruskal–Wallis and Dunn's tests at 5% significance level. Results The cytotoxicity assay revealed that multimethacrylate (Real Seal) was the most cytotoxic sealer ( P < 0.05) and exhibited the highest inflammatory potential against the SV 40 T‐Ag‐transfected cells ( P < 0.05). All root canal sealers tested were able to stimulate the immortalized pulp cells to produce IL ‐6, IL ‐8 and TNF ‐α, with differences in relation to the control group ( P < 0.05). Higher levels of IL ‐6, IL ‐8 and TNF ‐α were found in cell supernatant after stimulation with multimethacrylate (Real Seal) compared to all other sealers tested ( P < 0.05). No differences were found comparing epoxy resin‐based sealer ( AHP lus), single‐methacrylate sealer (Endo REZ ) and calcium hydroxide‐based sealer (Apexit Plus), regardless of the cytokine investigated (all P > 0.05). Conclusions A SV 40 T‐Ag‐transfected cell line of human pulp‐derived cells was established. The methacrylate resin‐based sealer (Real Seal) exhibited the greatest cytoxicity and inflammatory potential against immortalized pulp cells compared to an epoxy resin‐based sealer ( AH Plus), a methacrylate‐based sealer (EndoRez) and a calcium hydroxide‐based sealer (Apexit).