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QUANTIFICATION AND CHARACTERIZATION OF VIBRIO CHOLERAE ISOLATES FROM JAJU SAGAR DAM, NEEMUCH, (M.P.): A SEARCH FOR ESBL POSITIVE STRAIN

2014; Volume: 2014; Issue: 2 Linguagem: Inglês

2231-4490

Sonu Chouhan, Archana Pancholi, Asheeb Gupta,

Antibiotic Resistance in Bacteria

Here we present an analysis of the occurrence, enumeration, seasonal prevalence, characterization and ESBL status of Vibrio cholerae strains isolated from Jaju Sagar dam (raw water), Hingoria treatment plant (treated water) and Municipal tap (tap water). Seventy two samples (24 each of raw, treated and tap) were analyzed for total viable count of V. cholerae by Direct Plating Method using Thiosulphate Citrate Bile Salt Sucrose Agar. Sampling was done twice a month covering a period of 1 year (Jan 2013-Dec 2013).Results from the current study showed 100 % occurrence of V. cholerae in all the raw, treated and tap water samples, throughout the year. Significant (P<0.05) differences were observed in V. cholerae log counts among Raw, Treated and Tap water. Seasonal studies of raw water samples showed highest counts (P<0.05) in rainy season (July-Oct 2013) followed by summer (Mar-June 2013) and winter (Nov-Feb 2013). Throughout the whole investigation period no sample of treated and tap water complied with the drinking water standards laid down by WHO and BIS. All the tap water samples contained higher counts (P<0.05) than the treated water samples, throughout the year. These results suggest that water treatment plants in developing countries need to be monitored to ensure they achieve WHO acceptable levels of water quality. Another important aspect of our study was to determine the antibiotic susceptibility patterns of the isolates to frame Extended Spectrum β –lactamase (ESBL) detection. The study of antibiogram on 100 isolates revealed that all the tested strains (100%) were sensitive to tetracycline, imipenem and co-trimoxazole. 60% of the V. cholerae isolates were resistant to aztreonam,55% to cefuroxime,40% to cephotaxime,50% to ceftazidime, 35% to ceftriaxone, 25% to amoxyclav, 95% strains were sensitive and 5% were intermediate to cefoxitin. 60 strains, that were resistant to third generation cephalosporins were selected for further detection of the ESBL by Double Disc Synergy test but all these 60 strains gave negative Double Disc Synergy test hence no ESBL positive V.cholerae was detected in the present study. Due to the lack of a genetic analysis of these strains, the exact nature of the underlying mechanisms mediating the resistance is not clear.