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Identification and characterization of lactic acid bacteria isolated from rainbow trout ( Oncorhynchus mykiss, Walbaum 1792), with inhibitory activity against Vagococcus salmoninarum and Lactococcus garvieae

2017; Wiley; Volume: 24; Issue: 1 Linguagem: Inglês

10.1111/anu.12571

1365-2095

Behire Işıl Didinen, Ertan Emek Onuk, Seçil Metin, O. Cayli,

Aquaculture Nutrition and Growth

Aquaculture NutritionVolume 24, Issue 1 p. 400-407 ORIGINAL ARTICLE Identification and characterization of lactic acid bacteria isolated from rainbow trout (Oncorhynchus mykiss, Walbaum 1792), with inhibitory activity against Vagococcus salmoninarum and Lactococcus garvieae B.I. Didinen, Corresponding Author B.I. Didinen behiredidinen@hotmail.com orcid.org/0000-0003-2628-1480 Egirdir Fisheries Faculty, Suleyman Demirel University, Isparta, Turkey Correspondence Behire Isıl Didinen, Egirdir Fisheries Faculty, Suleyman Demirel University, Isparta, Turkey. Email: behiredidinen@hotmail.comSearch for more papers by this authorE.E. Onuk, E.E. Onuk Faculty of Veterinary Medicine, Department of Aquatic Animal Diseases, Ondokuz Mayıs University, Samsun, TurkeySearch for more papers by this authorS. Metin, S. Metin Egirdir Fisheries Faculty, Suleyman Demirel University, Isparta, TurkeySearch for more papers by this authorO. Cayli, O. Cayli Egirdir Fisheries Faculty, Suleyman Demirel University, Isparta, TurkeySearch for more papers by this author B.I. Didinen, Corresponding Author B.I. Didinen behiredidinen@hotmail.com orcid.org/0000-0003-2628-1480 Egirdir Fisheries Faculty, Suleyman Demirel University, Isparta, Turkey Correspondence Behire Isıl Didinen, Egirdir Fisheries Faculty, Suleyman Demirel University, Isparta, Turkey. Email: behiredidinen@hotmail.comSearch for more papers by this authorE.E. Onuk, E.E. Onuk Faculty of Veterinary Medicine, Department of Aquatic Animal Diseases, Ondokuz Mayıs University, Samsun, TurkeySearch for more papers by this authorS. Metin, S. Metin Egirdir Fisheries Faculty, Suleyman Demirel University, Isparta, TurkeySearch for more papers by this authorO. Cayli, O. Cayli Egirdir Fisheries Faculty, Suleyman Demirel University, Isparta, TurkeySearch for more papers by this author First published: 13 May 2017 https://doi.org/10.1111/anu.12571Citations: 14Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract In this study, a total of 98 lactic acid bacteria isolated from rainbow trout intestines were screened for their probiotic properties. The isolates were tested for their ability to inhibit growth of Vagococcus salmoninarum and Lactococcus garvieae. Based on in vitro antagonism, 10 isolates were selected and evaluated pathogenicity in rainbow trout. Isolates were further investigated for hydrophobicity, bile salts and acid tolerance. These isolates were able to survive low pH and high bile concentrations and showed good adherence characteristics. Isolates were characterized phenotypically, and then, 16S rRNA gene sequence analysis was used for confirmation. Selected strains were administered orally at 108 cfu/g feed, and fish were challenged with V. salmoninarum and L. garvieae. The fish fed with lactic acid bacteria supplemented diets did not improve protection against V. salmoninarum. However, administration of Lactococcus lactis subsp. lactis M17 2-2 and Lactobacillus sakei 2-3 resulted in a significant reduction in mortality due to L. garvieae when compared to the control fish. RPS values were calculated as 80 and 53% in fish fed with L. sakei 2-3 and L. lactis subsp. lactis M17 2-2, respectively. Our results suggest that these strains could provide an alternative for lactococcosis control in aquaculture. Citing Literature Volume24, Issue1February 2018Pages 400-407 RelatedInformation