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First Report of Sugarcane Leaf Scald Caused by Xanthomonas albilineans in the Province of Guangxi, China

2017; American Phytopathological Society; Volume: 101; Issue: 8 Linguagem: Inglês

10.1094/pdis-12-16-1774-pdn

1943-7692

R. Y. Zhang, Hongli Shan, W. F. Li, Xiao‐Yan Cang, X. Y. Wang, Jianhai Yin, Zhihong Luo, Ying‐Kun Huang,

Plant-Microbe Interactions and Immunity

HomePlant DiseaseVol. 101, No. 8First Report of Sugarcane Leaf Scald Caused by Xanthomonas albilineans in the Province of Guangxi, China PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Sugarcane Leaf Scald Caused by Xanthomonas albilineans in the Province of Guangxi, ChinaR. Y. Zhang, H. L. Shan, W. F. Li, X. Y. Cang, X. Y. Wang, J. Yin, Z. M. Luo, and Y. K. HuangR. Y. Zhang, H. L. Shan, W. F. Li, X. Y. Cang, X. Y. Wang, J. Yin, Z. M. Luo, and Y. K. HuangAffiliationsAuthors and Affiliations R. Y. Zhang H. L. Shan W. F. Li X. Y. Cang X. Y. Wang J. Yin Z. M. Luo Y. K. Huang , Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Yunnan Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan, 661699, China. Published Online:5 Jun 2017https://doi.org/10.1094/PDIS-12-16-1774-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Leaf scald caused by Xanthomonas albilineans (Ashby) Dowson is a destructive disease of sugarcane, as it causes severe economic losses (Rott et al. 1995). X. albilineans has been reported in over 60 countries in Africa, Asia, and North and South America (Rott et al. 2000), but has so far not been reported in mainland China. X. albilineans is a quarantine pathogen for sugarcane germplasm imported into China. In June 2016, symptoms of leaf scald were observed on the newly-bred sugarcane cultivars Guitang46 and Guitang06-2081 in Beihai, Laibin, and Baise, Guangxi Province, China. The average disease incidence of cultivars Guitang46 and Guitang06-2081 were 44.6 and 50.1%, respectively. Typical symptoms of the disease were white stripes along veins. Severely infected plants also exhibited shorter stalk internodes, smaller leaves, and abnormal development of side shoots along the stalk. Leaves of side shoots had similar white stripes as those observed at the top of the stalk. A sudden wilting of all leaves without any other symptoms was also observed for some diseased canes. Diseased plant samples from Beihai, Laibin, and Baise were collected. Circular, convex, smooth, shiny, yellow pigmented bacterial colonies were isolated from six symptomatic stalks from Beihai, Laibin, and Baise according to the method described by Davis et al. (1994). Total DNA were extracted from nine bacterial colonies and sugarcane juice from 21 infected plants, respectively, using a bacterial genomic DNA purification kit (Sangon Biotech Co., Ltd, Shanghai, China). About 600 bp PCR amplification products were obtained using specific primers XAF1/XAR1 for X. allbilineans (Wang et al. 1999). Thirty PCR products were subsequently cloned and sequenced. BLASTN analysis showed that the amplified nucleotide sequences from juice of infected plants (GenBank accession nos. KY315183–203) and from purified colonies (KY315204–209) shared 100% sequence identity with the nucleotide sequence of gene raxB1 of X. albilineans strain GPE PC73 (FP565176). To fulfill Koch's postulates, sugarcane cultivar Guitang46 was grown in a greenhouse and inoculated with suspensions of three bacterial isolates (108 CFU/ml), six plants per isolate, according to the method described by Rott et al. (2011). The inoculated plants were placed in a greenhouse at 24 to 35°C. One month post inoculation, symptoms of white stripes and wilting were observed on leaves of inoculated plants, but not on the six control plants treated with distilled water. The pathogen was reisolated from the symptomatic plants and identified as X. albilineans by PCR, sequencing, and sequence analysis. The pathogen was not detected in control plants. The disease was identified to be leaf scald caused by X. albilineans. To our knowledge, this is the first report of X. albilineans causing plant disease in mainland China. Because X. albilineans can be easily transmitted by vegetative propagation of sugarcane, leaf scald is a potential threat to the development of the sugar industry in China.References:Davis, M. J., et al. 1994. Plant Dis. 78:78. https://doi.org/10.1094/PD-78-0078 Crossref, ISI, Google ScholarRott, P., et al. 1995. Plant Pathol. 44:1075. https://doi.org/10.1111/j.1365-3059.1995.tb02667.x Crossref, ISI, Google ScholarRott, P., et al. 2000. Page 38 in: Leaf scald: A guide to sugarcane diseases. CIRAD Publication Services, Montepellier, France. Google ScholarRott, P., et al. 2011. Mol. Plant-Microbe Interact. 24:594. https://doi.org/10.1094/MPMI-07-10-0156 Link, ISI, Google ScholarWang, Z. K., et al. 1999. Plant Pathol. 48:245. https://doi.org/10.1046/j.1365-3059.1999.00332.x Crossref, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 101, No. 8 August 2017SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 12 Jul 2017Published: 5 Jun 2017First Look: 11 May 2017Accepted: 8 May 2017 Page: 1541 Information© 2017 The American Phytopathological SocietyCited bySugarcane responses to two strains of Xanthomonas albilineans differing in pathogenicity through a differential modulation of salicylic acid and reactive oxygen species15 December 2022 | Frontiers in Plant Science, Vol. 13Comparative genome analysis unravels pathogenicity of Xanthomonas albilineans causing sugarcane leaf scald disease26 September 2022 | BMC Genomics, Vol. 23, No. 1Isolation and Characterization of Rhizospheric Bacteria From Vuralia turcica Rhizospheric Soil29 May 2022 | Tarım Bilimleri DergisiXanthomonas albilineans (leaf scald of sugarcane)CABI Compendium, Vol. CABI CompendiumMolecular Detection and Quantification of Xanthomonas albilineans in Juice from Symptomless Sugarcane Stalks Using a Real-Time Quantitative PCR AssayYang Shi, Jian-Ying Zhao, Jing-Ru Zhou, Mbuya Sylvain Ntambo, Peng-Yuan Xu, Philippe C. 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