Produção Nacional
Revisado por pares
Annexin subcellular expression in laryngeal squamous cell carcinoma
2007; American Association for Cancer Research; Volume: 13; Linguagem: Inglês
ISSN
1557-3265
AutoresVenâncio Avancini Ferreira Alves, Suely Nonogaki, Patrícia Ramos Cury, Victor Wünsch‐Filho, Marcos De Bona de Carvalho, Pedro Michaluart, Raquel Ajub Moysés, Otávio Alberto Curioni, David Livingstone Alves Figueiredo, Cristovam Scapulatempo‐Neto, Edwin R. Parra, Wilson A. Silva, Francisco G. Nóbrega, Eloíza H. Tajara, Marco A. Zago,
Tópico(s)S100 Proteins and Annexins
ResumoA49 Annexin 1 is a soluble cytoplasmic protein, moving to membranes when calcium levels are elevated. Annexin 1 is also shown to move to nucleus or outside the cells, depending on tysosine-kinase signaling, thus interfering in cytoskeletal organization, ion fluxes and cell differentiation, mostly in inflammatory and neoplastic processes. In the present study, Sequencing Analysis of Gene Expression experiments detected a reduced expression of ANXA1 gene in laryngeal squamous cell carcinomas as compared to the corresponding non-neoplastic margins. Real time polymerase chain reaction confirmed ANXA1 low expression in another random subset of 15 larynx squamous cell carcinomas and 8 matched normal samples. Based on these data, we investigated subcellular patterns of immunohistochemical expression of annexin 1 in 259 paraffin-embedded samples from 95 patients with laryngeal squamous cell carcinomas, including non-tumoral, dysplastic, primary tumoral and lymphnode metastases areas, searching for evidences for the role of annexin 1 in laryngeal carcinogenesis. The results showed annexin 1 down-regulation in dysplastic, tumoral and metastatic lesions and provided preliminary evidence for the progressive migration of annexin 1 from the nucleus towards the membrane along the sequence of laryngeal carcinogenesis. Since this dysregulation was observed very early in carcinogenesis, already in intra-epithelial neoplasms, it was not found related to prognostic markers, such as nodal metastases.
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