Comparison of the cytotoxicity of formocresol, formaldehyde, cresol, and glutaraldehyde using human pulp fibroblast cultures.
1987; National Institutes of Health; Volume: 9; Issue: 4 Linguagem: Inglês
Autores
Huey-Wen Jeng, Robert J. Feigal, Harold H. Messer,
Tópico(s)Collagen: Extraction and Characterization
ResumoSerial dilution and agar overlay techniques were used to compare the cytotoxicity of formocresol, 19% formaldehyde, 35% cresol, and 2.5% glutaraldehyde to human pulp fibroblasts. The maximum nontoxic concentration of each agent was determined to allow quantitative comparisons both of the agents tested and of the techniques used. Formaldehyde was found to be the major component of formocresol which is responsible for the cytotoxic effect on human pulp fibroblasts. Two and one-half per cent glutaraldehyde was 15-20 times less toxic than either formocresol or 19% formaldehyde. Cresol measured 40 times less toxic than formaldehyde or formocresol. Both serial dilution and agar overlay techniques appear to be sensitive and effective methods for testing the toxicity of diffusible agents.
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