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Indigenous Indonesian Wild Silkworm Cocoon of Attacus atlas as Biocompatible Film Biomaterial

2017; IOP Publishing; Volume: 204; Linguagem: Inglês

10.1088/1757-899x/204/1/012011

1757-899X

Tjokorda Gde Tirta Nindhia, Zdeněk Knejzlı́k, Tomáš Ruml, I Wayan Surata, Tjok Sari Nindhia,

Insect Utilization and Effects

The biocompatible film made from wild silkworm cocoon of Attacus atlas is intorduced in this research for anticipation of demand on biocompatible film for regenerative medicine. The wild silkworm cocoon was indigenous Indonesia and was taken from it original location in Indonesia. Protocol for degumming method was obtained in this research by using treatment with NaOH solution at 0.1 M for 1 hour. The film was prepared by grinding the wet degummed fiber until pulp like state was obtained. The mixture was dropped in sequence on the hot ceramic plate with temperature around 60°C. The film thickness can be controlled precisely by using this technique. The film is soaked in alcohor for 1 day for stability testing and the result is found stable. The film is introduced in to COS-1 Cell suspension with previously washed in PBS solution and put in a chamber for biocompatibility testing. The cell are found able to grow and attach in first day observation and dramatically increase after 3 days observation. This is an indicate that the film that is produced from wild silkworm cocon of Attacus atlas has excellent biocompatibility.