First Report of Bell Pepper Soft-Rot Caused by Pectobacterium carotovorum subsp. brasiliense in Venezuela
2017; American Phytopathological Society; Volume: 101; Issue: 9 Linguagem: Inglês
10.1094/pdis-03-17-0361-pdn
ISSN1943-7692
AutoresAnnika Gillis, Marı́a Angélica Santana, María Inés Rodríguez, Gustavo Romay,
Tópico(s)Plant-Microbe Interactions and Immunity
ResumoHomePlant DiseaseVol. 101, No. 9First Report of Bell Pepper Soft-Rot Caused by Pectobacterium carotovorum subsp. brasiliense in Venezuela PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Bell Pepper Soft-Rot Caused by Pectobacterium carotovorum subsp. brasiliense in VenezuelaA. Gillis, M. A. Santana, M. Rodríguez, and G. RomayA. Gillishttp://orcid.org/0000-0002-6735-3359, M. A. Santana, M. Rodríguez, and G. RomayAffiliationsAuthors and Affiliations A. Gillis , Laboratory of Food and Environmental Microbiology, Earth and Life Institute, Université catholique de Louvain, 1348 Louvain-la-Neuve, Belgium M. A. Santana , Laboratorio de Biotecnología Agrícola, Departamento de Biología Celular, Universidad Simón Bolívar, Caracas, Venezuela M. Rodríguez , Laboratorio de Genética Molecular Bacteriana, Departamento de Biología Celular, Universidad Simón Bolívar, Caracas, Venezuela G. Romay , Applied Microbiology-Phytopathology, Earth and Life Institute, Université catholique de Louvain, 1348 Louvain-la-Neuve, Belgium. Published Online:3 Jul 2017https://doi.org/10.1094/PDIS-03-17-0361-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Soft rot symptoms were observed during the rainy season, particularly in July, in 2008 on bell pepper (Capsicum annuum L., hybrid RPP 1216, Syngenta) cultivated under greenhouse conditions (25/18°C, 85% RH) in Sartenejas, Miranda State, Venezuela. Initial symptoms on plants consisted of watery lesions at the basal part of the stem. As the lesions progressed, the whole stem presented water-soaked and necrotic tissue, with detached cortical tissues. Infected plants showed general wilt with defoliation. Fruits showed watery lesions that progressed until complete maceration. Plant samples displaying soft rot symptoms were collected to isolate the causal agent of the disease. Tissue from the lesions was surface-disinfected by standard protocols and ground to prepare serial dilutions that were plated on Luria Bertani-agar medium. After 48 h of incubation at 28°C, a predominant bacterium was isolated from all samples. Five strains from independent samples were subjected to biochemical and pathogenicity tests. All bacterial strains were gram-negative rods, motile, facultative anaerobic, nonfluorescent on King's B medium, oxidase-negative, catalase-positive, and grew at 28 and 37°C. Acid production was positive from arabinose, inositol, melibiose, and rhamnose, but negative from sorbitol. Bacterial strains formed pits on crystal violate pectate medium and induced a hypersensitive response in tobacco plants 24 h after inoculation with a 108 CFU/ml bacterial suspension in sterile phosphate buffered saline (PBS) solution. Strains were tested for tissue maceration on mature bell pepper fruits (Hybrid RPP 1216, Syngenta) by injecting 10 µl of the aforementioned bacterial suspension and incubating the fruits in a moist chamber at 28°C. All bacterial strains caused water-soaked lesions similar to those observed on fruits in greenhouse conditions. The first lesions appeared at 18 h and progressed rapidly until complete tissue maceration at 72 h. Since all the strains displayed similar symptom severity, along with identical phenotypic and biochemical characteristics, pathogenicity of one representative strain (AGPim1G) was further evaluated in bell pepper plants (Hybrid RPP 1216, Syngenta) cultured in vitro and in sterile soil as described by Gillis et al. (2014). Under in vitro conditions, watery lesions became visible at 24 h and severe maceration of the basal part of the stem was observed at 96 h. Symptoms formerly observed in greenhouses were also reproduced in planta. Bacteria with similar characteristics as those artificially inoculated were reisolated from the macerated tissues, completing Koch's postulates. No symptoms were observed on PBS-inoculated controls. The pel gene was PCR-amplified (434 bp) for all the strains using primers Y1/Y2 (Darrase et al. 1994), hence strains were identified as Pectobacterium spp. All five strains produced the 322-bp PCR amplicon with primers Br1f/L1r (Duarte et al. 2004), used for the detection of Pectobacterium carotovorum subsp. brasiliense (Pcb). The molecular identification of strain AGPim1G was further assessed. BLAST analysis of its partial 16S rRNA gene sequence (GenBank accession no. KY711431) showed 99% identity to that of Brazilian Pcb Type 1 strain 212T (JF926716). Pcb has been isolated in other Latin American countries such as Brazil, Peru, and Colombia (Duarte et al. 2004; Jaramillo et al. 2017; Nabhan et al. 2012). To our knowledge, this is the first report of Pcb infecting bell pepper in Venezuela.References:Darrase, A., et al. 1994. Appl. Environ. Microbiol. 60:1437. ISI, Google ScholarDuarte, V., et al. 2004. J. Appl. Microbiol. 96:535. https://doi.org/10.1111/j.1365-2672.2004.02173.x Crossref, ISI, Google ScholarGillis, A., et al. 2014. Eur. J. Plant Pathol. 138:1. https://doi.org/10.1007/s10658-013-0300-x Crossref, ISI, Google ScholarJaramillo, A., et al. 2017. Plant Dis. 101:830. https://doi.org/10.1094/PDIS-08-16-1184-PDN Link, ISI, Google ScholarNabhan, A. S., et al. 2012. J. Plant Pathol. 61:498. https://doi.org/10.1111/j.1365-3059.2011.02546.x Crossref, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 101, No. 9 September 2017SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 14 Aug 2017Published: 3 Jul 2017First Look: 25 May 2017Accepted: 23 May 2017 Pages: 1671-1671 Information© 2017 The American Phytopathological SocietyCited byControlling soft rot of postharvest chilli pepper (Capsicum annuum L.) by an antagonist Bacillus amyloliquefaciens S917: Efficacy and action modeBiological Control, Vol. 178Pectobacterium brasiliense (soft rot and blackleg of ornamentals and potato)CABI Compendium, Vol. CABI CompendiumPectobacterium carotovorumCABI Compendium, Vol. CABI CompendiumCharacterization of Pectobacterium brasiliense strains from potato and vegetables in Israel30 August 2021 | Plant Pathology, Vol. 70, No. 9Controlling pepper soft rot by Lactobacillus paracasei WX322 and identification of multiple bacteriocins by complete genome sequencingFood Control, Vol. 121Pectobacterium brasiliense: Genomics, Host Range and Disease Management5 January 2021 | Microorganisms, Vol. 9, No. 1Diseases Caused by Pectobacterium and Dickeya Species Around the World5 January 2021Characterization of bacterial pathogens causing fruit soft rot and stem blight of bell pepper in Georgia, USA6 December 2019 | Journal of Plant Pathology, Vol. 102, No. 2Pectobacteriumcarotovorum subsp. brasiliense Causes Soft Rot and Death of Neobuxbaumia tetetzo in Zapotitlan Salinas Valley, Puebla, MexicoDimas Mejía-Sánchez, Sergio Aranda-Ocampo, Cristian Nava-Díaz, Daniel Teliz-Ortiz, Manuel Livera-Muñoz, Rodolfo De La Torre-Almaráz, and Samuel Ramírez-Alarcón9 January 2019 | Plant Disease, Vol. 103, No. 3Transfer of the waterfall source isolate Pectobacterium carotovorum M022 to Pectobacterium fontis sp. nov., a deep-branching species within the genus PectobacteriumInternational Journal of Systematic and Evolutionary Microbiology, Vol. 69, No. 2The Changing Face of Bacterial Soft-Rot DiseasesAnnual Review of Phytopathology, Vol. 56, No. 1Pectobacterium carotovorum subsp. brasiliense and Pectobacterium carotovorum subsp. carotovorum as causal agents of potato soft rot in Algeria21 February 2018 | European Journal of Plant Pathology, Vol. 151, No. 4
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