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Occurrence of Cowpea mild mottle virus in Common Bean and Associated Weeds in Northeastern Brazil

2017; American Phytopathological Society; Volume: 101; Issue: 10 Linguagem: Inglês

10.1094/pdis-04-17-0562-pdn

ISSN

1943-7692

Autores

N. S. Lamas, V. O. R. L. Matos, D. M. T. Alves-Freitas, Fernando L. Melo, Antônio Félix da Costa, Josías C. Faria, Simone G. Ribeiro,

Tópico(s)

Agricultural pest management studies

Resumo

HomePlant DiseaseVol. 101, No. 10Occurrence of Cowpea mild mottle virus in Common Bean and Associated Weeds in Northeastern Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseOccurrence of Cowpea mild mottle virus in Common Bean and Associated Weeds in Northeastern BrazilN. S. Lamas, V. O. R. L. Matos, D. M. T. Alves-Freitas, F. L. Melo, A. F. Costa, J. C. Faria, and S. G. RibeiroN. S. LamasSearch for more papers by this author, V. O. R. L. MatosSearch for more papers by this author, D. M. T. Alves-FreitasSearch for more papers by this author, F. L. MeloSearch for more papers by this author, A. F. CostaSearch for more papers by this author, J. C. Fariahttp://orcid.org/0000-0002-8125-7654Search for more papers by this author, and S. G. Ribeiro†Corresponding author. E-mail: E-mail Address: simone.ribeiro@embrapa.brSearch for more papers by this authorAffiliationsAuthors and Affiliations N. S. Lamas V. O. R. L. Matos D. M. T. Alves-Freitas , Embrapa Recursos Genéticos e Biotecnologia, Brasília, Brazil F. L. Melo , Universidade de Brasília, Brazil A. F. Costa , Instituto Agronômico de Pernambuco, Recife, Brazil J. C. Faria , Embrapa Arroz e Feijão, Goiânia, Brazil S. G. Ribeiro † , Embrapa Recursos Genéticos e Biotecnologia, Brasília, Brazil. Published Online:16 Aug 2017https://doi.org/10.1094/PDIS-04-17-0562-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Cowpea mild mottle virus (CPMMV - Carlavirus, Betaflexiviridae) is a positive single-stranded RNA virus transmitted by the whitefly Bemisia tabaci (Adams et al. 2012). Common bean is a staple food in Brazil, and virus-associated diseases can cause important crop losses. The first report of CPMMV in Brazil was in common bean (Phaseolus vulgaris L.) in the states of São Paulo and Paraná, causing angular yellow mosaic (Costa et al. 1983). More recently, CPMMV has re-emerged and was frequently found infecting beans in Goiás State and Distrito Federal, located in central Brazil. However, in the northeastern region, CPMMV infection in common bean plants has not been reported. During a virus survey in common bean and associated uncultivated plants in experimental fields, plants presenting virus-like symptoms such as yellow mosaic, leaf deformation, and leaf curling were collected in plots at the Instituto Agronomico de Pernambuco experimental stations in Arcoverde, Caruaru, and Ibimirim in August 2013. Total RNA was extracted, and cDNA was synthesized with M-MLV reverse transcription using random octamers and oligo-dT primers. PCR reactions were performed using CPMMV specific primers CPMMV-4000F (5′-AACTTGGCCTTAGTGAACTCTACA-3′) and CPMMV-4500R (5′-ATTAGCTCTGTGCCTGGGGT-3′). Expected fragments of 500 bp were amplified, and PCR products from selected isolates were cloned into PCRII-TOPO-TA vector and sequenced to confirm the identity of the virus. A high incidence of CPMMV infection in beans was observed in all locations with 80% (4/5) of virus-positive samples in Ibimirim, 92.3% (12/13) in Caruaru, and 100% (21/21) in Arcoverde, updating the geographical distribution of CPMMV in beans in Brazil. The CPMMV amplicon sequences recovered from bean plants (GenBank KY930609–KY930611) were nearly identical and shared 99% identity with the corresponding sequence of a CPMMV isolate from whiteflies from Florida (KC774019.1) (Rosario et al. 2014), and 98% identical to a CPMMV isolate from soybean from Brazil (KC884246.1) (Zanardo et al. 2014). CPMMV was also detected in Sida sp. (Malvaceae), confirming the earlier observations made by Costa et al. (1983), and for the first time CPMMV was found in uncultivated plants belonging to several botanical families, including Fabaceae (Macroptilium spp., Senna spp., Desmodium glabrum, Rhynchosia minima), Nyctaginaceae (Mirabilis jalapa), Cleomaceae (Cleome affinis), and Asteraceae (Blainvillea rhomboidea). The CPMMV amplicons recovered from these uncultivated plants (KY930606–KY930608 and KY930612–KY930618) shared an identity of 96.8 to 99% with those retrieved from beans. This is the first report of CPMMV found in common bean and a wide range of uncultivated hosts in the state of Pernambuco, Brazil. Our results show the importance of monitoring re-emerging viral pathogens such as CPMMV in both common bean and uncultivated plants as increase in the incidence, host range, and geographical distribution of CPMMV brings a new perspective to the study and development of common bean cultivars resistant to this virus.References:Adams, M. J., et al. 2012. Page 920 in: Ninth Report of the International Committee on Taxonomy of Viruses. Elsevier Academic Press, New York. Google ScholarCosta, A. S., et al. 1983. Fitopatol. Bras. 8:325. Google ScholarRosario, K., et al. 2014. PLoS One 9:e86748. https://doi.org/10.1371/journal.pone.0086748 Crossref, ISI, Google ScholarZanardo, L. G., et al. 2014. Plant Pathol. 63:456. https://doi.org/10.1111/ppa.12092 Crossref, ISI, Google ScholarThis study was supported by grants from Embrapa, CNPq and FAPDF. N. S. Lamas and D. M. T. Alves-Freitas are recipients of fellowships from CNPq.DetailsFiguresLiterature CitedRelated Vol. 101, No. 10 October 2017SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 14 Sep 2017Published: 16 Aug 2017First Look: 31 May 2017Accepted: 28 May 2017 Pages: 1828-1828 Information© 2017 The American Phytopathological SocietyCited byOccurrence of bean-associated cytorhabdovirus in soybean fields in BrazilCrop Protection, Vol. 163Cowpea mild mottle virus, a sometimes problem for French bean crops21 September 2022 | Australasian Plant Pathology, Vol. 51, No. 6Synonymous Codon Pattern of Cowpea Mild Mottle Virus Sheds Light on Its Host Adaptation and Genome Evolution30 March 2022 | Pathogens, Vol. 11, No. 4Cowpea mild mottle virus (angular mosaic of beans)CABI Compendium, Vol. CABI CompendiumNext-Generation Sequencing-Based Detection of Common Bean Viruses in Wild Plants from Tanzania and Their Mechanical Transmission to Common Bean PlantsBeatrice Mwaipopo, Minna-Liisa Rajamäki, Neema Ngowi, Susan Nchimbi-Msolla, Paul J. R. Njau, Jari P. T. Valkonen, and Deusdedith R. Mbanzibwa24 October 2021 | Plant Disease, Vol. 105, No. 9Diversity and Distribution of Viruses Infecting Wild and Domesticated Phaseolus spp. in the Mesoamerican Center of Domestication16 June 2021 | Viruses, Vol. 13, No. 6Population Dynamics of Whiteflies and Associated Viruses in South America: Research Progress and Perspectives28 November 2020 | Insects, Vol. 11, No. 12A survey of RNA genome viruses in Lima bean crops of Northeastern Brazil1 September 2020 | Bragantia, Vol. 79, No. 3Transmission of the Bean-Associated Cytorhabdovirus by the Whitefly Bemisia tabaci MEAM115 September 2020 | Viruses, Vol. 12, No. 9First report of Yambean mosaic virus in Brazil11 July 2019 | Australasian Plant Disease Notes, Vol. 14, No. 1Vigna unguiculata (Cowpea)6 June 2020Phaseolus vulgaris (Common bean/French bean/Snap bean)6 June 2020

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