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Carbon nanotube field effect transistor biosensor for the detection of toxins in seawater

2017; Taylor & Francis; Volume: 97; Issue: 7 Linguagem: Inglês

10.1080/03067319.2017.1334056

1029-0397

Inês M. C. Marques, João Pinto da Costa, Celine I.L. Justino, Patrícia S.M. Santos, Kátia Duarte, Ana C. Freitas, Susana Cardoso, Armando C. Duarte, Teresa Rocha‐Santos,

Analytical Chemistry and Sensors

Disposable field effect transistors (FET) biosensors (bio-FET) based on carbon nanotubes were fabricated for detection of domoic acid (DA), which belongs to the group of biotoxins associated with the amnesic shellfish poisoning. The analytical results obtained with the bio-FET were compared with those obtained with a traditional methodology (enzyme-linked immunosorbent assay) in order to validate the bio-FET for DA detection. Standard solutions of DA with concentrations between 10 and 500 ng L−1 were tested in order to construct the calibration curve, where five bio-FET were used for reproducibility estimation and two analytical measurements were performed for each bio-FET for repeatability estimation. Ten spiked artificial seawater samples were used to validate the bio-FET. The obtained reproducibility (0.52–1.43%), repeatability (0.57–1.27%), limit of detection (10 ng L−1) and recovery range (92.3–100.3%) reveal an adequate analytical performance of the bio-FET for the detection of DA in environmental samples such as seawater samples.