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Protein–Sol: a web tool for predicting protein solubility from sequence

2017; Oxford University Press; Volume: 33; Issue: 19 Linguagem: Inglês

10.1093/bioinformatics/btx345

1367-4811

Max Hebditch, Manuel Alejandro Carballo‐Amador, Spyros Charonis, Robin Curtis, Jim Warwicker,

Bacterial Genetics and Biotechnology

Protein solubility is an important property in industrial and therapeutic applications. Prediction is a challenge, despite a growing understanding of the relevant physicochemical properties.Protein-Sol is a web server for predicting protein solubility. Using available data for Escherichia coli protein solubility in a cell-free expression system, 35 sequence-based properties are calculated. Feature weights are determined from separation of low and high solubility subsets. The model returns a predicted solubility and an indication of the features which deviate most from average values. Two other properties are profiled in windowed calculation along the sequence: fold propensity, and net segment charge. The utility of these additional features is demonstrated with the example of thioredoxin.The Protein-Sol webserver is available at http://protein-sol.manchester.ac.uk.jim.warwicker@manchester.ac.uk.