Produção Nacional
Revisado por pares
Patterns of expression of odorant receptor genes in a Chagas disease vector
2015; Elsevier BV; Volume: 69; Linguagem: Inglês
10.1016/j.ibmb.2015.05.002
ISSN1879-0240
AutoresJosé Manuel Latorre-Estivalis, Emerson Soares de Oliveira, Barbara Beiral Esteves, Letícia Santos Guimarães, Marina Neves Ramos, Marcelo Gustavo Lorenzo,
Tópico(s)Invertebrate Immune Response Mechanisms
ResumoRhodnius prolixus is a triatomine bug acting as a relevant vector of Chagas disease for which the genome sequence has been recently made available. Based on this information, a set of olfactory (ORs) and ionotropic receptor (IRs) genes potentially related to olfactory processes was characterized, and the expression patterns along bug development and in different structures potentially involved in promoting chemosensory-mediated behaviors were studied. For this, diverse bioinformatic procedures were used to validate gene models analyzing their structural and functional features and designing specific primers. Evolutionary relationships among R. prolixus olfactory coreceptors (RproOrco, RproIR25a, RproIR8a and RproIR76b) and their orthologues from other insects were shown to have mostly good bootstrap support values in phylogenetic trees. Moreover, antennal expression was confirmed for most genes included in the study. Both ORs and IRs showed antennal expression along the whole development of bugs of this species, with few exceptional receptors showing gradually increasing expression or expression restricted to the antennae of adult bugs. Finally, the expression of most of the selected genes was confirmed in other structures, such as rostri, tarsi, tibial pads and genitalia, which are potentially involved in promoting chemosensory-mediated behaviors. These results are discussed in terms of their relevance to advance in the understanding of the molecular bases of triatomine behavior.
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