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First Report of Bipolaris micropus , Curvularia geniculata , Epicoccum sorghinum , and Fusarium incarnatum on Paspalum guenoarum Seeds in Rio Grande do Sul, Brazil
2017; American Phytopathological Society; Volume: 101; Issue: 9 Linguagem: Romeno
10.1094/pdis-12-16-1746-pdn
ISSN1943-7692
AutoresBruno Frosi Gasparetto, Lúcia Brandão Franke, Camila Cristina Lage de Andrade, M. Dalbosco, Vinícius da Silva Duarte, Silvino Intra Moreira, Eduardo Alves,
Tópico(s)Mycorrhizal Fungi and Plant Interactions
ResumoHomePlant DiseaseVol. 101, No. 9First Report of Bipolaris micropus, Curvularia geniculata, Epicoccum sorghinum, and Fusarium incarnatum on Paspalum guenoarum Seeds in Rio Grande do Sul, Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Bipolaris micropus, Curvularia geniculata, Epicoccum sorghinum, and Fusarium incarnatum on Paspalum guenoarum Seeds in Rio Grande do Sul, BrazilB. F. Gasparetto, L. B. Franke, C. C. L. Andrade, M. Dalbosco, V. Duarte, S. I. Moreira, and E. AlvesB. F. GasparettoSearch for more papers by this author, L. B. FrankeSearch for more papers by this author, C. C. L. Andrade†Corresponding author. E-mail: E-mail Address: camilalage86@gmail.comSearch for more papers by this author, M. DalboscoSearch for more papers by this author, V. DuarteSearch for more papers by this author, S. I. MoreiraSearch for more papers by this author, and E. AlvesSearch for more papers by this authorAffiliationsAuthors and Affiliations B. F. Gasparetto L. B. Franke , Department of Forage Plants and Agrometeorology, UFRGS, Porto Alegre, RS, 91540-000, Brazil C. C. L. Andrade † M. Dalbosco V. Duarte , Agronômica - Laboratório de Diagnóstico Fitossanitário e Consultoria, Porto Alegre, RS, 91530-000, Brazil S. I. Moreira E. Alves , Department of Plant Pathology, UFLA, Lavras, MG, 37200000, Brazil. Published Online:17 Jul 2017https://doi.org/10.1094/PDIS-12-16-1746-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Paspalum guenoarum Arechav. is a tropical forage species that occurs naturally in South America. In May 2015, P. guenoarum plants (virgule) showing a large number of lesions, ranging from elliptical to elongated, with brown coloration, covering more than 50% of the leaf area, were observed in the field plots located in Eldorado do Sul, RS, Brazil. Seeds from those plants showed a high incidence of various fungi. Subsamples of 100 seeds were submitted to the blotter test at 20°C with a 12-h photoperiod for 7 days (MAPA 2009). After isolation on potato dextrose agar (PDA) culture medium, structures of the following fungi were observed under a microscope: conidia (32 to 57 × 16 to 21 μm) of Bipolaris sp., conidia (17 to 25 × 12 to 19 μm) of Curvularia sp.; in carnation leaf agar (CLA) medium, canoe-shaped or banana-shaped mesoconidia (9 to 16 × 2 to 3 μm) of Fusarium sp., and pycnidia (100 to 250 × 105 to 240 μm in diam.) and conidia (8 to 12 × 5 to 7 μm) of Phoma sp. The isolates were deposited in the Mycological Collection of the Federal University of Lavras (UFLA), Lavras, MG, as CML 3600 (Bipolaris micropus), CML 3602 (Curvularia geniculata), CML 3599 (Epicoccum sorghinum), and CML 3601 (Fusarium incarnatum). The species were identified based on the rDNA internal transcribed spacer (ITS) (primers ITS1/ITS4; White et al. 1990). The RNA polymerase II (RPB2) gene (primers 5F2/7cR; O’Donnell et al. 2007) was used to identify Fusarium sp. The ITS region showed 99% identity to the isolate CML 3600 (GenBank accession no. KY20355) of B. micropus (HE792935.1), CML 3602 (KY120356) was 99% identical to Cochliobolus geniculatus (KF590040.1), which is the obsolete teleomorph name of Curvularia geniculata. Isolate CML 3599 (KY120357) was 100% identical to E. sorghinum (KY454467.1). Using the RPB2 gene, CML 3601 (KY120358) was found to be 99% identical to F. incarnatum (KF255542.1). A total of 60 seedlings were inoculated by spraying (1 ml/seedling) a spore suspension (1 × 105 CFU/ml) with each of the isolates over both the adaxial and abaxial leaf surfaces. Sixty additional plants were sprayed with water as the control. The plants were incubated at 22°C with a 12:12 h light/dark cycle. After 10 days, all inoculated seedlings developed necrotic lesions, except the controls. The pathogens were recovered from the infected tissues, fulfilling the Koch’s postulates. This is the first report of incidence of B. micropus, C. geniculata, E. sorghinum, and F. incarnatum associated with P. guenoarum seeds in Brazil. Further investigations are warranted to determine if one or more of these fungi are responsible for low seed germination rate (76%) of the P. guenoarum seeds.References:MAPA. 2009. Manual de Análise Sanitária de Sementes. Ministério da Agricultura, Pecuária e Abastecimento, Brasilia, Brazil. Google ScholarO’Donnell, K., et al. 2007. J. Clin. Microbiol. 45:2235. https://doi.org/10.1128/JCM.00533-07 Crossref, ISI, Google ScholarWhite, T. J., et al. 1990. Page 315 in: PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego. Google ScholarDetailsFiguresLiterature CitedRelated Vol. 101, No. 9 September 2017SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 14 Aug 2017Published: 17 Jul 2017First Look: 5 Jun 2017Accepted: 1 Jun 2017 Pages: 1679-1679 Information© 2017 The American Phytopathological SocietyCited bySeed-borne diseases in pasture grasses and legumes: state of the art and gaps in knowledge31 December 2022 | Journal of Plant Diseases and Protection, Vol. 18Epicoccum spp. Causing Maize Leaf Spot in Heilongjiang Province, ChinaXi Xu, Jingjing Li, Xilang Yang, Li Zhang, Shuo Wang, Guijin Shen, Bing Hui, Jialei Xiao, Changjian Zhou, Xiangjing Wang, Junwei Zhao, and Wensheng Xiang21 November 2022 | Plant Disease, Vol. 106, No. 12Sequence Data, Functional Annotation, and Relationship Analysis Between mRNAs and Long Noncoding RNAs from Tea Leaves During Infection by the Fungal Pathogen Epicoccum sorghinumChen Huang, Hongke Huang, Zhongqiu Xia, Yuqin Yang, Xinyue Jiang, Yuanyou Yang, Delu Wang, Xiangyang Li, and Zhuo Chen15 September 2022 | Molecular Plant-Microbe Interactions, Vol. 35, No. 9Endophytic fungi from an overlooked plant species: A case study in Kelissa brasiliensis (Baker) Ravenna1 January 2022 | Acta Botanica Brasilica, Vol. 36Epicoccum species: ubiquitous plant pathogens and effective biological control agents22 January 2021 | European Journal of Plant Pathology, Vol. 159, No. 4Fungi associated with Paspalum guenoarum seeds: their impact on physiology and control1 January 2021 | Ciência Rural, Vol. 51, No. 9Sanitary quality of seed of Paspalum species1 September 2020 | Ornamental Horticulture, Vol. 26, No. 3A polyphasic approach to delineate species in Bipolaris25 May 2020 | Fungal Diversity, Vol. 102, No. 1First Report of Leaf Spot of Maize (Zea mays) Caused by Curvularia geniculata in ChinaD. 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