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First Report of Phytophthora infestans Self-Fertile Genotypes in Southern Brazil
2017; American Phytopathological Society; Volume: 101; Issue: 9 Linguagem: Inglês
10.1094/pdis-02-17-0215-pdn
ISSN1943-7692
AutoresV. H. Casa-Coila, Miller S. Lehner, B. T. Hora Júnior, Ailton Reis, Nilceu Ricetti Xavier de Nazareno, Eduardo S. G. Mizubuti, C. B. Gomes,
Tópico(s)Peanut Plant Research Studies
ResumoHomePlant DiseaseVol. 101, No. 9First Report of Phytophthora infestans Self-Fertile Genotypes in Southern Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Phytophthora infestans Self-Fertile Genotypes in Southern BrazilV. H. Casa-Coila, M. S. Lehner, B. T. Hora Júnior, A. Reis, N. R. X. Nazareno, E. S. G. Mizubuti, and C. B. GomesV. H. Casa-Coila†Corresponding author. E-mail: E-mail Address: [email protected]Search for more papers by this author, M. S. LehnerSearch for more papers by this author, B. T. Hora JúniorSearch for more papers by this author, A. ReisSearch for more papers by this author, N. R. X. NazarenoSearch for more papers by this author, E. S. G. MizubutiSearch for more papers by this author, and C. B. GomesSearch for more papers by this authorAffiliationsAuthors and Affiliations V. H. Casa-Coila † , Programa de Pós-Graduação em Fitossanidade, Universidade Federal de Pelotas, Pelotas, 96010-610, Rio Grande do Sul, Brazil M. S. Lehner , Programa de Pós-Graduação em Genética e Melhoramento, Universidade Federal de Viçosa, 36570-900, Viçosa, Minas Gerais, Brazil B. T. Hora Júnior , Monsanto do Brasil Ltda, Uberlândia, 38407049, Minas Gerais, Brazil A. Reis , Embrapa Hortaliças CEP 70351-970, Brasília, DF, Brazil N. R. X. Nazareno , Instituto Agronômico do Paraná, Curitiba, 82630-900, Paraná, Brazil E. S. G. Mizubuti , Departamento de Fitopatologia, Universidade Federal de Viçosa, 36570-900, Viçosa, Minas Gerais, Brazil C. B. Gomes , Embrapa Clima Temperado, Pelotas, 96010-971, Rio Grande do Sul, Brazil. Published Online:17 Jul 2017https://doi.org/10.1094/PDIS-02-17-0215-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Late blight, caused by Phytophthora infestans, is the most devastating potato disease worldwide. The pathogen is a heterothallic species, and therefore individuals of different types (A1 and A2) must mate for sexual reproduction to take place (Fry et al. 1993). However, some isolates of P. infestans are homothallic and can produce oospores in the absence of A1 and A2 mating types (Orona et al. 2013). Some fungicides also can induce self-production of oospores in heterothallic isolates of P. infestans (Groves and Ristaino 2000). Self-fertile isolates have increased fitness, greater aggressiveness, and can be more resistant to fungicides than isolates from the A1 mating type (Zhu et al. 2016). The occurrence of P. infestans self-fertile isolates may increase the risk of sexual reproduction and consequently increase the genotypic diversity in the pathogen population (Han et al. 2013). During the autumn of 2011 and 2012, leaf and stem samples of potato plants infected with P. infestans were collected in southern Brazil. Monosporangial isolates of P. infestans were obtained from the samples and mating types were determined. Each isolate was paired with the A2 and A1 mating types of P. infestans and self-paired on Petri plates containingV8 juice agar. After this, the plates were kept at 18°C in darkness for 20 days. Three isolates collected in Paraná State (municipalities of Ponta Grossa 25°12′26″S, 50°07′11″W; Contenda 25°38′52″S, 49°34′26″W; and Castro 24°51′50″S, 49°53′50″W) formed oospores when paired with both mating type isolates and also when self-paired. Many oospores were produced from each pairing. Furthermore, the inoculation of these three self-fertile isolates on detached leaflets of cultivar Ágata (cultivated in greenhouse) reproduced disease symptoms and produced oospores. The SSR genotypes of three self-fertile and 47 heterothallic isolates were determined based on nine loci: G11, Pi02, Pi89, Pi04, Pi70, Pi56(Long), Pi63(Long), Pi33, and D13. The forward primers were labeled with one of the three fluorescent dyes: NED (Pi33, Pi89, and G11), FAM (Pi02, Pi70, D13, and Pi63(Long)), or HEX (Pi04, Pi56(Long)). PCR products were sequenced to determine the allele at each locus. In five (Pi02, Pi33, D13, Pi04, and G11) out of nine loci, allele sizes were different between self-fertile and heterothallic isolates. In these five loci for the self-fertile isolates, three loci were heterozygous: 146/160 bp in locus Pi02, 200/203 bp in Pi33, and 138/140 bp in D13. The Pi04 locus was homozygous monomorphic with 168/168 bp. The G11 locus was homozygous with 158/158 bp allele. Self-fertility in P. infestans isolates is a known phenomenon but this is the first report of self-fertile isolates obtained from field samples of potato in Brazil. This information is important for planning strategies for management of potato late blight in the Southern region of Brazil.References:Fry, W. E., et al. 1993. Plant Dis. 77:653. https://doi.org/10.1094/PD-77-0653 Crossref, ISI, Google ScholarGroves, C. T., and Ristaino, J. B. 2000. Phytopathology 90:1201. https://doi.org/10.1094/PHYTO.2000.90.11.1201 Link, ISI, Google ScholarHan, M., et al. 2013. J. Eukaryot. Microbiol. 60:79. https://doi.org/10.1111/jeu.12010 Crossref, ISI, Google ScholarOrona, C. L., et al. 2013. Plant Dis. 97:1112. https://doi.org/10.1094/PDIS-10-12-0962-PDN Link, ISI, Google ScholarZhu, W., et al. 2016. Sci. Rep. 6:29428. https://doi.org/10.1038/srep29428 Crossref, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 101, No. 9 September 2017SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 14 Aug 2017Published: 17 Jul 2017First Look: 8 Jun 2017Accepted: 3 Jun 2017 Pages: 1682-1682 Information© 2017 The American Phytopathological SocietyCited byThe contributions of potassium phosphite and hormonal pathways to the control of Phytophthora infestans in tomato cv. 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