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Intraoperative assessment of tumor margins during glioma resection by desorption electrospray ionization-mass spectrometry

2017; National Academy of Sciences; Volume: 114; Issue: 26 Linguagem: Inglês

10.1073/pnas.1706459114

1091-6490

Valentina Pirro, Clint M. Alfaro, Alan K. Jarmusch, Eyas M. Hattab, Aaron Cohen‐Gadol, R. Graham Cooks,

Nanopore and Nanochannel Transport Studies

Significance Gliomas infiltrate into surrounding healthy brain tissue. Microsurgical resection aims for maximal tumor resection while minimizing morbidity. Surgical margins are defined based on the surgeon’s experience, visual observation, and neuronavigation. Surgical margin assessment is rarely undertaken intraoperatively due to time constraints and unreliability of such evaluation. Routine, pathologic intraoperative examination provides no molecular information. Molecular measurements using mass spectrometry can be made rapidly on tissue during surgery to identify tissue types, estimate tumor infiltration, and recognize the presence of prognostic mutations by monitoring oncometabolites and phospholipids. This intraoperative study demonstrates the power of mass spectrometry in assessing diagnostic and prognostic information on discrete surgeon-defined points along the resection margins to improve tumor resection, even in regions without MRI contrast enhancement.