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Molecular Determinants for Cyclo-Oligosaccharide-Based Nanoparticle-Mediated Effective siRNA Transfection

2017; Future Medicine; Volume: 12; Issue: 13 Linguagem: Inglês

10.2217/nnm-2017-0123

1748-6963

Darío Manzanares, Ingrid Araya‐Durán, Laura Gallego‐Yerga, Pablo Játiva, Valeria Márquez‐Miranda, Jonathan Canan, José L. Jiménez Blanco, Carmen Ortiz Mellet, Fernando D. González‐Nilo, José M. Garcı́a Fernández, Valentı́n Ceña,

DNA and Nucleic Acid Chemistry

To study the structural requirements that a cyclooligosaccharide-based nanoparticle must fulfill to be an efficient siRNA transfection vector.siRNA protection from degradation by RNAses, transfection efficiency and the thermodynamic parameters of the nanoparticle/siRNA interactions were studied on pairs of amphiphilic molecules using biochemical techniques and molecular dynamics.The lower the siRNA solvent accessible surface area in the presence of the nanoparticle, higher the protection from RNAse-mediated degradation in the corresponding nanocomplex; a moderate nanoparticle/siRNA binding energy value further facilitates reversible complexation and binding to the target cellular mRNA.The use, in advance, of these parameters will provide a useful indication of the potential of a molecular nanoparticle as siRNA transfecting vector.