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Synthesis, DNA Cleavage Activity, Cytotoxicity, Acetylcholinesterase Inhibition, and Acute Murine Toxicity of Redox‐Active Ruthenium(II) Polypyridyl Complexes

2017; Wiley; Volume: 12; Issue: 13 Linguagem: Inglês

10.1002/cmdc.201700240

1860-7187

Nagham Alatrash, Eugenia Soyo Narh, Abhishek Yadav, Mahn‐Jong Kim, Thamara Janaratne, James Gabriel, Frederick M. MacDonnell,

Ferrocene Chemistry and Applications

Abstract Four mononuclear [(L‐L) 2 Ru(tatpp)] 2+ and two dinuclear [(L‐L) 2 Ru(tatpp)Ru(L‐L) 2 ] 4+ ruthenium(II) polypyridyl complexes (RPCs) containing the 9,11,20,22‐tetraazatetrapyrido[3,2‐a:2′,3′‐c:3′′,2′′‐l:2′′′,3′′′‐ n ]pentacene (tatpp) ligand were synthesized, in which L‐L is a chelating diamine ligand such as 2,2′‐bipyridine (bpy), 1,10‐phenanthroline (phen), 3,4,7,8‐tetramethyl‐1,10‐phenanthroline (Me 4 phen) or 4,7‐diphenyl‐1,10‐phenanthroline (Ph 2 phen). These Ru–tatpp analogues all undergo reduction reactions with modest reducing agents, such as glutathione (GSH), at pH 7. These, plus several structurally related but non‐redox‐active RPCs, were screened for DNA cleavage activity, cytotoxicity, acetylcholinesterase (AChE) inhibition, and acute mouse toxicity, and their activities were examined with respect to redox activity and lipophilicity. All of the redox‐active RPCs show single‐strand DNA cleavage in the presence of GSH, whereas none of the non‐redox‐active RPCs do. Low‐micromolar cytotoxicity (IC 50 ) against malignant H358, CCL228, and MCF7 cultured cell lines was mainly restricted to the redox‐active RPCs; however, they were substantially less toxic toward nonmalignant MCF10 cells. The IC 50 values for AChE inhibition in cell‐free assays and the acute toxicity of RPCs in mice revealed that whereas most RPCs show potent inhibitory action against AChE (IC 50 values <15 μ m ), Ru–tatpp complexes as a class are surprisingly well tolerated in animals relative to other RPCs.