The Deep-Sea Polyextremophile Halobacteroides lacunaris TB21 Rough-Type LPS: Structure and Inhibitory Activity towards Toxic LPS
2017; Multidisciplinary Digital Publishing Institute; Volume: 15; Issue: 7 Linguagem: Inglês
10.3390/md15070201
ISSN1660-3397
AutoresFlaviana Di Lorenzo, Angelo Palmigiano, Ida Paciello, Mateusz Pallach, Domenico Garozzo, Maria Lina Bernardini, Violetta La Cono, Michail M. Yakimov, Antonio Molinaro, Alba Silipo,
Tópico(s)S100 Proteins and Annexins
ResumoThe structural characterization of the lipopolysaccharide (LPS) from extremophiles has important implications in several biomedical and therapeutic applications. The polyextremophile Gram-negative bacterium Halobacteroides lacunaris TB21, isolated from one of the most extreme habitats on our planet, the deep-sea hypersaline anoxic basin Thetis, represents a fascinating microorganism to investigate in terms of its LPS component. Here we report the elucidation of the full structure of the R-type LPS isolated from H. lacunaris TB21 that was attained through a multi-technique approach comprising chemical analyses, NMR spectroscopy, and Matrix-Assisted Laser Desorption Ionization (MALDI) mass spectrometry. Furthermore, cellular immunology studies were executed on the pure R-LPS revealing a very interesting effect on human innate immunity as an inhibitor of the toxic Escherichia coli LPS.
Referência(s)