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Specific CD45 isoforms differentially regulate T cell receptor signaling.

1994; Springer Nature; Volume: 13; Issue: 4 Linguagem: Inglês

10.1002/j.1460-2075.1994.tb06322.x

1460-2075

Daniel Chui, Christopher J. Ong, Pauline Johnson, H S Teh, Jamey D. Marth,

Signaling Pathways in Disease

Research Article15 February 1994free access Specific CD45 isoforms differentially regulate T cell receptor signaling. D. Chui D. Chui Biomedical Research Centre, University of British Columbia, Vancouver, Canada. Search for more papers by this author C.J. Ong C.J. Ong Biomedical Research Centre, University of British Columbia, Vancouver, Canada. Search for more papers by this author P. Johnson P. Johnson Biomedical Research Centre, University of British Columbia, Vancouver, Canada. Search for more papers by this author H.S. Teh H.S. Teh Biomedical Research Centre, University of British Columbia, Vancouver, Canada. Search for more papers by this author J.D. Marth J.D. Marth Biomedical Research Centre, University of British Columbia, Vancouver, Canada. Search for more papers by this author D. Chui D. Chui Biomedical Research Centre, University of British Columbia, Vancouver, Canada. Search for more papers by this author C.J. Ong C.J. Ong Biomedical Research Centre, University of British Columbia, Vancouver, Canada. Search for more papers by this author P. Johnson P. Johnson Biomedical Research Centre, University of British Columbia, Vancouver, Canada. Search for more papers by this author H.S. Teh H.S. Teh Biomedical Research Centre, University of British Columbia, Vancouver, Canada. Search for more papers by this author J.D. Marth J.D. Marth Biomedical Research Centre, University of British Columbia, Vancouver, Canada. Search for more papers by this author Author Information D. Chui1, C.J. Ong1, P. Johnson1, H.S. Teh1 and J.D. Marth1 1Biomedical Research Centre, University of British Columbia, Vancouver, Canada. The EMBO Journal (1994)13:798-807https://doi.org/10.1002/j.1460-2075.1994.tb06322.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Multiple isoforms of T cell CD45 tyrosine phosphatase are expressed as a result of alternative RNA splicing among extracellular exons. To discern the presence and identity of distinct functions among CD45 isoforms, we compared thymic T cell activation responses by elevating expression of two CD45 isoforms normally found on quiescent T cells. We report that CD45RABC significantly increased CD4+ thymic T cell proliferation in both a mixed lymphocyte reaction and following anti-T cell receptor (TCR) antibody stimulation. Additionally, CD45RABC enhanced Ca2+ mobilization and phosphotyrosine accumulation, and suppressed the inhibitory effect of anti-CD4 antibodies. By contrast, CD45R0 did not enhance TCR signaling or phosphotyrosine levels in CD4+ thymic T cells and required a TCR co-stimulus to augment cellular proliferation. These studies provide genetic evidence that alternative CD45 isoforms are functionally distinct and disclose a unique mechanism by which T cell immunologic responsiveness can be modified. Previous ArticleNext Article Volume 13Issue 41 February 1994In this issue RelatedDetailsLoading ...