Portal de Periódicos da CAPES

Glycosaminoglycan Analysis by Cryogenic Messenger-Tagging IR Spectroscopy Combined with IMS-MS

2017; American Chemical Society; Volume: 89; Issue: 14 Linguagem: Inglês

10.1021/acs.analchem.7b01467

1520-6882

Neelam Khanal, Chiara Masellis, Michael Z. Kamrath, David E. Clemmer, Thomas R. Rizzo,

Mass Spectrometry Techniques and Applications

We combine ion mobility spectrometry with cryogenic, messenger-tagging, infrared spectroscopy and mass spectrometry to identify different isomeric disaccharides of chondroitin sulfate (CS) and heparan sulfate (HS), which are representatives of two major subclasses of glycosaminoglycans. Our analysis shows that while CS and HS disaccharide isomers have similar drift times, they can be uniquely distinguished by their vibrational spectrum between ∼3200 and 3700 cm–1 due to their different OH hydrogen-bonding patterns. We suggest that this combination of techniques is well suited to identify and characterize glycan isomers directly, which presents tremendous challenges for existing methods.