Produção Nacional
An improved protocol for in vitro propagation of the medicinal plant Mimosa pudica L.
2017; Academic Journals; Volume: 16; Issue: 9 Linguagem: Inglês
10.5897/ajb2016.15831
ISSN1684-5315
AutoresRicardo Bianchetti, Ferrara de Resende Cristiano, Vinícius S. Pacheco, Floriano Dornellas Felipe, Mystica Silva de Oliveira Aline, Jober Condé Evangelista Freitas, Henrique Pereira Peixoto Paulo,
Tópico(s)Plant Parasitism and Resistance
ResumoThis work aimed to develop a protocol for the in vitro establishment, multiplication, rooting and ex vitro acclimatization of Mimosa pudica L., a species used in folk medicine and with pharmacological activity. Aseptic cultures were established from seeds inoculated in MS medium, without growth regulators, followed by an in vitro stabilization phase in culture medium supplemented with 2.22 μM BAP. The cultures were transferred to MS medium supplemented with different cytokinins, combined or not with an auxin, aiming its large-scale propagation. The culture medium supplemented with 5 µM BAP plus 0.5 µM NAA provided the highest multiplication rate and top quality plantlets. The combination of 0.6 µM TDZ plus 0.05 µM NAA resulted in higher multiplication rates than in response to combination of BAP plus NAA, although the subsequent maintenance of the cultures in a medium without growth regulators has resulted in low regenerative response. In vitro rooting of micro-cuttings was high even in the absence of auxins. Over 90% of plantlets transferred to the greenhouse survived after the acclimatization phase. Acclimatized plants presented normal vegetative and reproductive development. The procedures established in the present study allow a massive production of M. pudica plants for further pharmacological studies. Key words: Biodiversity conservation, ex vitro acclimatization, in vitro rooting, micropropagation, Mimosa pudica.
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