Studies on the Effects of Water Extract from Mixture of Pine Needles, Sedum sarmentosum Bunge, Hijkiaorme, Buckwheat and Perlla Leaves on the Immune Function Activation
2008; The Korean Society of Food and Nutrition; Volume: 21; Issue: 3 Linguagem: Inglês
ISSN
2287-4992
Autores Tópico(s)Chromatography in Natural Products
ResumoAbstract Plants have long been used as a food source in Korea. In this study, we investigated the combined immunomodulative effects of a water extract mixture of(pine needles, Sedum sarmentosum Bunge, hijkiaorme, buckwheat and Perlla leaves) on Balb/c mice 7 ～ 8 weeks old. The mice were fed a chow diet ad libitum and the plant extract was orally administered every other day for four weeks at two different concentrations(50 and 500 ㎎/㎏ BW). After preparing the single-cell suspension, splenocyte proliferation was determined by the MTT(3-[4,5-di-methylthiazol-2-y]-2,5-diphenyl terazolium bromide) assay. After 48hrs of incubation with the mitogens(ConA or LPS) splenocyte from the mice groups administered 50 and 500 ㎎/㎏ BW of the plant extract showed a significant increased in proliferation compared to the control group. A hemolytic plague forming cell assay was used to indicate antibody production against sheep red blood cells(SRBC). The number of antibody-secreting cells T-dependent antigen. The result of this study suggest that supplementation with this plant extract may regulate immune function by increasing splenocyte proliferation and the number of plaque forming cells. Key words: mixture, splenocyte proliferation, SRBC, immune.
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